NdhV Is a Subunit of NADPH Dehydrogenase Essential for Cyclic Electron Transport in Synechocystis sp Strain PCC 6803
NdhV Is a Subunit of NADPH Dehydrogenase Essential for Cyclic Electron Transport in Synechocystis sp Strain PCC 6803
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NdhV 是集胞藻 (Synechocystis sp.) 中循环电子传递所必需的 NADPH 脱氢酶亚基。
DOI：
10.1104/pp.15.01430
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发表时间：
2016-02-01
期刊：
影响因子：
7.4
通讯作者：
Ma, Weimin
中科院分区：
文献类型：
--
作者：
Gao, Fudan;Zhao, Jiaohong;Ma, Weimin
从蓝细菌集胞藻（Synechocystis sp.）菌株PCC 6803中分离到两个对热胁迫敏感的突变体，它们对NADPH脱氢酶（NDH-1）依赖的围绕光系统I的循环电子传递（NDH-CET）敏感。两个突变体在相同的sll 0272基因中具有标签，编码与在拟南芥（Arabidopsis thaliana）中鉴定的NdhV高度同源的蛋白。sll 0272基因的缺失（ndhV）不影响NDH-1复合物的组装以及CO2吸收和呼吸的活性，但降低了NDH-CET的活性。NdhV与蓝藻NDH-1复合物的铁氧还蛋白结合亚基NdhS相互作用。NdhS的缺失使NdhV完全消失，但NdhV的缺失对NdhS的量没有影响。NDH-CET活性的降低在Delta ndhS中比在Delta ndhV中更显著。因此，我们建议，NdhV与NdhS合作，接受还原铁氧还蛋白的电子。
Two mutants sensitive to heat stress for growth and impaired in NADPH dehydrogenase (NDH-1)-dependent cyclic electron transport around photosystem I (NDH-CET) were isolated from the cyanobacterium Synechocystis sp. strain PCC 6803 transformed with a transposon-bearing library. Both mutants had a tag in the same sll0272 gene, encoding a protein highly homologous to NdhV identified in Arabidopsis (Arabidopsis thaliana). Deletion of the sll0272 gene (ndhV) did not influence the assembly of NDH-1 complexes and the activities of CO2 uptake and respiration but reduced the activity of NDH-CET. NdhV interacted with NdhS, a ferredoxin-binding subunit of cyanobacterial NDH-1 complex. Deletion of NdhS completely abolished NdhV, but deletion of NdhV had no effect on the amount of NdhS. Reduction of NDH-CET activity was more significant in Delta ndhS than in Delta ndhV. We therefore propose that NdhV cooperates with NdhS to accept electrons from reduced ferredoxin.