FORMATION OF AN ION-TRANSPORT SUPERCOMPLEX IN ESCHERICHIA-COLI - AN EXPERIMENTAL-MODEL OF DIRECT TRANSDUCTION OF ENERGY
FORMATION OF AN ION-TRANSPORT SUPERCOMPLEX IN ESCHERICHIA-COLI - AN EXPERIMENTAL-MODEL OF DIRECT TRANSDUCTION OF ENERGY
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DOI：
10.1016/0014-5793(89)80272-8
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发表时间：
1989-03-27
期刊：
影响因子：
3.5
通讯作者：
MARTIROSOV, SM
中科院分区：
文献类型：
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作者：
BAGRAMYAN, KA;MARTIROSOV, SM
在厌氧生长的E中，观察到在ATP驱动的H+/K+交换期间发生氢气产生。在需氧培养的细胞、在硝酸盐培养基上生长的厌氧细胞、降低渗透压或去除K+以及最后应用DCCD、砷酸盐或CCCP时，均未发现这两个过程。二硫苏糖醇恢复的过程中，即使在存在的CCCP，但在其他情况下的抑制。提出了一个多酶转运超复合体模型。该超复合体由三个遗传上独立的机制组成：F0 F1 H +-ATPase提供能量，K+-Trk系统作为能量汇，甲酸氢裂解酶作为还原当量供体，能量的直接传递是通过2 SH氧化成S-S完成的。
Hydrogen gas production was observed to occur during ATP-driven H+/K+exchange in anaerobically grownE. coli.Neither process was found in aerobically grown cells or anaerobic cells grown on nitrate medium or when the osmotic pressure was decreased or K+removed, or finally when DCCD, arsenate or CCCP was applied. Dithiothreitol restored the process even in the presence of CCCP but not in other cases of inhibition. A model of a multienzyme transport supercomplex is proposed. The supercomplex consists of three genetically independent mechanisms: F0F1H+-ATPase to provide energy, the K+-transporting Trk system as energy sink and formate-hydrogen lyase as donor of reducing equivalents.Within this supercomplex direct transduction of energy is accomplished via oxidation of 2 SH to S-S.