Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.
Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.
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DOI：
10.18632/aging.101200
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发表时间：
2017-03-18
期刊：
影响因子：
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通讯作者：
Wang HY
中科院分区：
文献类型：
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作者：
Yang XZ;Cui SZ;Zeng LS;Cheng TT;Li XX;Chi J;Wang R;Zheng XF;Wang HY
Rab1B最近被报道与人类癌症有关，但Rab1B在结直肠癌（CRC）中的作用仍不清楚。本研究采用qRT-PCR、免疫印迹和免疫组化方法检测Rab1B和MMP 9在大肠癌中的表达，并分析其临床意义。结果表明，Rab1B和MMP 9在CRC细胞系和组织中的mRNA和蛋白水平均增加，如通过qRT-PCR和免疫印迹所测量的。Rab1B和MMP 9在179例结直肠癌组织中的高表达与肿瘤浸润深度、淋巴结转移和TNM分期有关。生存分析表明Rab1B或MMP 9过表达的患者的总生存期和无进展生存期明显低于低表达者，但对化疗的反应优于低表达者，Rab1B是结直肠癌患者的独立预后因素。此外，当Rab1B和MMP 9结合成一个新的风险模型时，它比单独使用每种蛋白质具有更好的预后预测。总之，Rab1B和MMP 9是潜在的预后生物标志物，它们的组合显著提高了CRC患者生存和化疗反应的预测能力。
Rab1B has recently been reported to be involved in human cancer, but the role of Rab1B in colorectal cancer (CRC) remains unclear. In this study, we investigated the expression of Rab1B and MMP9 in CRC by qRT-PCR, immunoblot and immunohistochemistry and analyzed the clinical significance. The results show that Rab1B and MMP9 are increased at both mRNA and protein levels in CRC cell lines and tissues, as measured by qRT-PCR and immunoblotting. The high protein expression of Rab1B and MMP9 in 179 CRC tissues is associated with deep tumor invasion, lymph-node metastasis and advanced TNM stage. Survival analysis indicates that patients with overexpression of Rab1B or MMP9 have significantly worse overall survival and progression-free survival, but better response to chemotherapy than those with low expression of proteins, and that Rab1B is an independent prognostic factor for CRC patients. Furthermore, when Rab1B and MMP9 are combined into a new risk model, it has a remarkably better prediction of prognosis than each protein alone. In conclusion, Rab1B and MMP9 are potential prognostic biomarkers and their combination significantly improves predictive power for survival and chemotherapy response in CRC patients.