Bioimaging for Study of the Myocardial Function: Fluorescence Microscopy Featuring the Na<sup>+</sup>-Ca<sup>2+</sup> Exchanger
Bioimaging for Study of the Myocardial Function: Fluorescence Microscopy Featuring the Na<sup>+</sup>-Ca<sup>2+</sup> Exchanger
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用于研究心肌功能的生物成像：采用 Na<sup> </sup>-Ca<sup>2 </sup> 交换器的荧光显微镜
DOI：
10.11169/bioimages.30.1
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Hikaru Tanaka
中科院分区：
文献类型：
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作者：
Namekata Iyuki;Ryosuke Odaka;Shogo Hamaguchi;Hikaru Tanaka
在这篇综述中，我们概述了我们对心肌Na+- ca2 +交换器的研究。Na+- ca2 +交换器是一种位于肌膜上的反向转运蛋白，可以催化3个Na+和1个ca2 +的电交换，无论是ca2 +外流（正向）还是ca2 +内流（反向）模式。首先，通过荧光显微镜和动力学分析对Na+- ca2 +交换剂的抑制剂进行了评价。其次，通过快速扫描共聚焦显微镜观察细胞内ca2 +振荡，阐明前向模式Na+- ca2 +交换剂在心肌兴奋中的作用。第三，用各种荧光探针证实了反向模式Na+- ca2 +交换器参与心肌缺血和再灌注时的细胞损伤。我们的研究得到了生物成像技术与电生理学和药理学相结合的支持。生物成像是现代生物学中一种强有力的方法。通过一些谨慎和努力来优化技术以满足研究需求，生物成像将为所有研究人员带来丰硕成果。
In this review, we provide an overview of our studies on the myocardial Na+-Ca 2+ exchanger, an antiporter located on the sarcolemma that catalyzes the electrogenic exchange of three Na+ and one Ca 2+, either in the Ca 2+ efflux (forward) or Ca 2+ influx (reverse) modes. Firstly, an inhibitor of the Na+-Ca 2+ exchanger was evaluated by fluorescence microscopy and a kinetic analysis. Secondly, intracellular Ca 2+ oscillations were observed by rapid scanning confocal microscopy and the role of the forward-mode Na+-Ca 2+ exchanger in myocardial excitation was clarified. Thirdly, the involvement of the reverse-mode Na+-Ca 2+ exchanger in cellular damage during myocardial ischemia and reperfusion was demonstrated with various fluorescent probes. Our research was supported by bioimaging technology in combination with electrophysiology and pharmacology. Bioimaging is a powerful methodology in modern biology. With some caution and effort to optimize the technology to meet research needs, bioimaging would be fruitful for all researchers.