Keratinocytes become terminally differentiated in a process involving programmed cell death
Keratinocytes become terminally differentiated in a process involving programmed cell death
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DOI：
10.1006/bbrc.1997.7405
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发表时间：
1997-09-29
影响因子：
3.1
通讯作者：
Toyoshima, K
中科院分区：
文献类型：
--
作者：
Maruoka, Y;Harada, H;Toyoshima, K
口腔角质形成细胞起源于前基底细胞，在迁移到口腔表面的过程中分化，最后脱落。细胞凋亡发生在分化末期，但分化终末与细胞凋亡之间的确切关系尚不清楚。在本研究中，Bcl-xL在基底细胞和棘细胞层表达，Bax在棘细胞和颗粒细胞层表达。在培养的角质形成细胞中，Bcl-xL在0.1 mM钙（低Ca2+）条件下表达，但在1.0 mM钙（高Ca2+）条件下消失；后者诱导角质形成细胞分化。 Bax 在低 Ca2+ 的角质形成细胞中不表达，但在高 Ca2+ 的细胞中表达。最终，高Ca2+的角质形成细胞发生凋亡，通过TUNEL方法和180-bp DNA片段化检测。这些结果表明牙龈上皮的终末分化过程是细胞凋亡的途径。 (C) 1997 年学术出版社。
Oral keratinocytes originate front basal cells, differentiate during migration to tate surface, and finally are shed. Apoptosis occurs at the end of differentiation, but the precise relationship between terminal differentiation and apoptosis is not clear. In the present study, Bcl-xL was expressed in the basal cell and spinous cell layers, and Bax was expressed in the spinous cell and granular cell layers. In cultured keratinocytes, Bcl-xL was expressed under conditions of 0.1 mM calcium (low Ca2+) but disappeared under conditions of 1.0 mM calcium (high Ca2+); the latter induces keratinocyte differentiation. Bax was not expressed in keratinocytes with low Ca2+ but was expressed in cells with high Ca2+. Finally keratinocytes with high Ca2+ underwent apoptosis, which was detected by the TUNEL method and by 180-bp DNA fragmentation. These results suggest that the process of terminal differentiation in gingival epithelium is a pathway to apoptosis. (C) 1997 Academic Press.