Comparison of selection strategies for marker-assisted backcrossing of a gene
Comparison of selection strategies for marker-assisted backcrossing of a gene
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DOI：
10.2135/cropsci1999.3951295x
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发表时间：
1999-09-01
期刊：
影响因子：
2.3
通讯作者：
Melchinger, AE
中科院分区：
文献类型：
--
作者：
Frisch, M;Bohn, M;Melchinger, AE
标记辅助选择可以加速回交育种中轮回亲本基因组(RPG)的恢复。在这项研究中，我们使用计算机模拟来比较关于(I)回收的RPG的比例和(Ii)回交程序中所需的标记数据点(MDP)的选择策略，该回交程序旨在将一个目标等位基因从供体品系导入到受体品系中。用已发表的玉米(Zea Mays L.)由80个标记组成的遗传图谱。目标等位基因的选择是基于表型评价。与所有世代的恒定种群规模相比，从Bc1代到BC3代种群规模的增加使所需的MDP数量减少了50%，而不影响RPG的比例。四阶段选择方法在第一代中强调对目标等位基因载体染色体上的重组子的选择，与考虑整个基因组的所有标记的选择指数相比，所需的MDP数量减少了75%。采用上述原则设计分子标记辅助回交计划，大大节省了所需的MDP数量。
Marker-assisted selection can accelerate recovery of the recurrent parent genome (RPG) in backcross breeding. In this study, we used computer simulations to compare selection strategies with regard to (i) the proportion of the RPG recovered and (ii) the number of marker data points (MDP) required in a backcross program designed for introgression of one target allele from a donor line into a recipient line. Simulations were performed with a published maize (Zea mays L.) genetic map consisting of 80 markers. Selection for the target allele was based on phenotypic evaluation. In comparison to a constant population size across all generations, increasing population sizes from generation BC1 to BC3 reduced the number of required MDP by as much as 50% without affecting the proportion of the RPG. A four-stage selection approach, emphasizing in the first generations selection for recombinants on the carrier chromosome of the target allele, reduced the required number of MDP by as much as 75% in comparison to a selection index taking into account all markers across the genome. Adopting the above principles for the design of marker-assisted backcross programs resulted in substantial savings in the number of MDP required.