Toll-like receptor 9 is expressed on follicle-associated epithelia containing M cells in swine Peyer's patches
Toll-like receptor 9 is expressed on follicle-associated epithelia containing M cells in swine Peyer's patches
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DOI：
10.1016/j.imlet.2004.10.026
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发表时间：
2005-04-15
影响因子：
4.4
通讯作者：
Saito, T
中科院分区：
文献类型：
--
作者：
Shimosato, T;Tohno, M;Saito, T
Toll 样受体 (TLR) 9 在肠道相关淋巴组织 (GALT) 中的精确分布和表达尚未阐明。在这项研究中。我们通过实时定量 PCR、蛋白质印迹、共聚焦激光显微镜和流式细胞术分析研究了成年和新生猪 GALT 中 TLR9 的表达模式。猪的 TLR9 基因优先在成年派尔氏淋巴结 (Pps) 和肠系膜淋巴结 (MLN) 中表达，其中 TLR9 含量大约是脾脏的三倍。其他组织仅表现出 TLR9 的弱表达。在新生猪中，仅在 MLN 中检测到 TLR9 表达升高。我们首先证明了在 Pps 和 MLN 中形成了高表达（TLR9（+））细胞。此外，TLR9(+)细胞不仅存在于树突状细胞和B细胞等免疫细胞中，而且还存在于Pps中的膜细胞(M细胞)等滤泡相关上皮细胞(FAE)中。这些结果表明，Pps 和 MLN 为宿主防御提供了在初始免疫反应的传导位点对来自细菌的各种生物活性寡核苷酸 (ODN) 做出反应的能力。 (c) 2004 Elsevier B.V 保留所有权利。
The precise distribution and expression of Toll-like receptor (TLR) 9 in gut-associated lymphoid tissues (GALTs) has not been elucidated. In this study. we investigated the expression pattern of TLR9 in adult and neonatal swine GALTs by real-time quantitative PCR, western blot, confocal laser microscopy and flow cytometric analysis. The swine TLR9 gene was preferentially expressed in adult Peyer's patches (Pps) and mesenteric lymph nodes (MLNs), which contained approximately three times higher TLR9 than the spleen. Other tissues exhibited only weak expression of TLR9. In neonatal swine, elevated expression of TLR9 was detected only in MLNs. We firstly showed that highly expressive (TLR9(+)) cells were formed in Pps and MLNs. In addition, TLR9(+) cells were present not only in immune cells such as dendritic cells and B cells but also in follicle-associated epithelia (FAE) including membranous cells (M cells) in Pps. These results suggest that Pps and MLNs provide the host defense with the ability to respond to a variety of bioactive oligonucleotides (ODNs) from bacteria at a conductive site of initial immune responses. (c) 2004 Elsevier B.V All rights reserved.