Nanoparticle-Mediated X-Ray Radiation Enhancement for Cancer Therapy.
Nanoparticle-Mediated X-Ray Radiation Enhancement for Cancer Therapy.
复制标题
纳米粒子介导的 X 射线辐射增强癌症治疗。
DOI：
10.1007/978-1-4939-6646-2_25
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
vandeVen,AnneL
中科院分区：
文献类型：
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作者：
Paro,AutumnD;Shanmugam,Ilanchezhian;vandeVen,AnneL
Metallic nanoparticles with a high atomic number release Auger electrons in response to external beam X-ray radiation. When these nanoparticles are selectively delivered to tumors, they have the potential to locally enhance the effects of radiation therapy. Optimizing the therapeutic efficacy of these nanoparticles, however, remains a challenging and time-consuming task. Here we describe three different assays that can be used to experimentally quantify and optimize the in vitro therapeutic efficacy of nanoparticle-mediated X-ray radiation enhancement. These include an IC50 extended dose response curve, clonogenic cell survival assay, and immunoblotting. Collectively, these assays provide information about whether a given nanoparticle provides radiosensitization, the extent of the radiosensitization, and the potential mechanism of radiosensitization.
