Raman/absorption simultaneous measurements for cytochrome oxidase compound A at room temperature with a novel flow apparatus.
Raman/absorption simultaneous measurements for cytochrome oxidase compound A at room temperature with a novel flow apparatus.
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使用新型流动装置在室温下同时测量细胞色素氧化酶化合物 A 的拉曼/吸收。
DOI：
10.1021/bi00446a008
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
T. Kitagawa
中科院分区：
文献类型：
--
作者：
T. Ogura;S. Yoshikawa;T. Kitagawa
建立了一种新型的连续生产细胞色素氧化酶反应中间体的流动装置，并成功地应用于观察细胞色素氧化酶与氧反应的瞬态吸收和共振拉曼光谱。在500-650-nm区域中的时间分辨差吸收光谱清楚地表明，在5 ° C下完全还原的CO结合形式光解后形成化合物A，并且在该阶段电子没有从细胞色素c转移到细胞色素氧化酶。然而，在化合物B的形成阶段，细胞色素c被氧化。同时还报道了这些中间体的共振拉曼光谱和吸收光谱。
A novel flow apparatus for continuously producing reaction intermediates of cytochrome oxidase was constructed and applied successfully to observe the transient absorption and resonance Raman spectra in its reaction with oxygen. Time-resolved difference absorption spectra in 500-650-nm region clearly indicated the formation of compound A upon photolysis of the fully reduced CO-bound form at 5 degrees C, and at this stage electrons were not transferred from cytochrome c to cytochrome oxidase. However, at the stage of formation of compound B, cytochrome c was oxidized. Resonance Raman spectra of these intermediates measured simultaneously with the absorption spectra are also reported.