ADIPSIN - A CIRCULATING SERINE PROTEASE HOMOLOG SECRETED BY ADIPOSE-TISSUE AND SCIATIC-NERVE
ADIPSIN - A CIRCULATING SERINE PROTEASE HOMOLOG SECRETED BY ADIPOSE-TISSUE AND SCIATIC-NERVE
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DOI：
10.1126/science.3299705
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发表时间：
1987-07-24
期刊：
影响因子：
56.9
通讯作者：
SPIEGELMAN, BM
中科院分区：
文献类型：
--
作者：
COOK, KS;MIN, HY;SPIEGELMAN, BM
Adipsin是一种丝氨酸蛋白酶同系物，其一级结构是从分化依赖性脂肪细胞信使RNA的核苷酸序列预测的。用合成肽的抗血清探测的免疫印迹鉴定了由3T3脂肪细胞合成和分泌的两种形式的adipsin。这些44和37千道尔顿的蛋白质通过酶促去糖基化转化为25.5千道尔顿。虽然脂蛋白酶主要在脂肪组织中合成，但它也由坐骨神经产生，并在血液中发现。由于脂蛋白酶的合成明显局限于在脂质代谢中高度活跃的组织，其存在于血清中，并且其在改变的代谢状态中的调节，该分子可能在全身脂质代谢或能量平衡中起先前未被认识到的作用。
Adipsin is a serine protease homolog whose primary structure was predicted from the nucleotide sequence of a differentiation-dependent adipocyte messenger RNA. Immunoblots probed with antisera to synthetic peptides identify two forms of adipsin that are synthesized and secreted by 3T3 adipocytes. These proteins of 44 and 37 kilodaltons are converted to 25.5 kilodaltons by enzymatic deglycosylation. Although adipsin is principally synthesized in adipose tissue, it is also produced by sciatic nerve and is found in the bloodstream. Because of the apparent restriction of adipsin synthesis to tissues highly active in lipid metabolism, its presence in serum, and its modulation in altered metabolic states, this molecule may play a previously unrecognized role in systemic lipid metabolism or energy balance.