Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source
Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source
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DOI：
10.1117/1.2193428
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Tromberg, Bruce J.
中科院分区：
文献类型：
--
作者：
Tang, Shuo;Krasieva, Tatiana B.;Tromberg, Bruce J.
一个12 fs的宽带（100 nm）的来源是用来联合收割机多光子显微镜（MPM）和光学相干层析成像（OCT）在一个单一的平台。一个超快钛：蓝宝石激光器同时提供短脉冲所需的有效MPM激发和高分辨率OCT所需的宽带宽。使用0.3 μ m的微球和63倍，0.95数值孔径的物镜，我们证明，MPM和OCT通道共注册的横向分辨率约0.5 μ m和轴向分辨率约1.5 μ m。一个3-D器官型上皮组织模型的初步研究表明，荧光和二次谐波信号的多光子图像分别来自细胞和细胞外基质结构，而OCT图像产生的散射界面，由于组织的折射率的变化。结合MPM/OCT显微镜是能够提供同时从细胞和细胞外基质的功能和结构信息，是一个潜在的强大的工具，用于研究厚组织中的生物过程。(c)2006年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
A 12-fs broadband (100-nm) source is used to combine multiphoton microscopy (MPM) and optical coherence tomography (OCT) in a single platform. An ultrafast Ti:sapphire laser simultaneously provides short pulses necessary for efficient MPM excitation and the broad bandwidth required for high-resolution OCT. Using 0.3-mu m microspheres and a 63X, 0.95 numerical aperture objective, we demonstrate that MPM and OCT channels are coregistered with lateral resolution of approximately 0.5 mu m and axial resolution of approximately 1.5 mu m. Preliminary studies of a 3-D organotypic epithelial tissue model show that multiphoton images of fluorescence and second harmonic signals are derived from cellular and extracellular matrix structures, respectively, while OCT images are generated from scattering interfaces due to tissue variations in refractive index. The combined MPM/OCT microscope is capable of providing simultaneous functional and structural information from cells and extracellular matrix and is potentially a powerful tool for studying biological processes in thick tissues. (c) 2006 Society of Photo-Optical Instrumentation Engineers.