Changes in the expression of the carbohydrate epitope HNK-1 associated with mesoderm induction in the chick embryo.
Changes in the expression of the carbohydrate epitope HNK-1 associated with mesoderm induction in the chick embryo.
复制标题
与鸡胚中胚层诱导相关的碳水化合物表位 HNK-1 表达的变化。
作者：
D. R. Canning;C. Stern
我们报告的单克隆抗体，HNK-1，确定特定的区域和细胞类型在鸡胚盘原条形成。免疫组织化学研究表明，形成的下胚层细胞是HNK-1阳性的，从最早的时候，他们可以被识别。胚盘边缘的一些细胞也呈阳性。原条的中胚层细胞从其最初出现时起就被抗体强烈染色。在上胚层中，一些细胞在原始条纹前阶段是阳性的，一些是阴性的，但是随着原始条纹的发育，在上层内观察到染色强度的梯度，朝向原始条纹增加。在发育的后期，脊索和头褶的间充质是阳性的，而其余的中胚层（侧板）不再表达HNK-1免疫反应性。因此，该抗体揭示了与中胚层诱导相关的变化：在诱导前，它识别“诱导”组织（下胚层）并揭示了响应组织（上胚层）中的马赛克模式;在原条形成后，由诱导相互作用产生的原条的中胚层强烈表达表位。对各种组织中的HNK-1相关蛋白进行亲和纯化，然后通过SDS-PAGE对其进行鉴定。HNK-1相关蛋白在原肠形成期鸡胚的下胚层、中胚层和上胚层中存在一些共同的蛋白，而另一些蛋白则是特定组织所特有的。已经尝试使用蛋白质印迹和已知识别HNK-1相关分子的抗体来鉴定这些蛋白质，但是所使用的抗体都不能鉴定所研究的任何组织所特有的条带。我们的结论是，这些蛋白质可能是HNK-1/L2家族的新成员，它们可能在早期发育过程中的细胞相互作用中发挥作用。
We report that a monoclonal antibody, HNK-1, identifies specific regions and cell types during primitive streak formation in the chick blastoderm. Immunohistochemical studies show that the cells of the forming hypoblast are HNK-1 positive from the earliest time at which they can be identified. Some cells of the margin of the blastoderm are also positive. The mesoderm cells of the primitive streak stain strongly with the antibody from the time of their initial appearance. In the epiblast, some cells are positive and some negative at pre-primitive-streak stages, but as the primitive streak develops a gradient of staining intensity is seen within the upper layer, increasing towards the primitive streak. At later stages of development, the notochord and the mesenchyme of the headfold are positive, while the rest of the mesoderm (lateral plate) no longer expresses HNK-1 immunoreactivity. This antibody therefore reveals changes associated with mesodermal induction: before induction, it recognizes the 'inducing' tissue (the hypoblast) and reveals a mosaic pattern in the responding tissue (the epiblast); after primitive streak formation, the mesoderm of the primitive streak that results from the inductive interactions expresses the epitope strongly. Affinity purification of HNK-1-related proteins in various tissues was carried out, followed by SDS-PAGE to identify them. The hypoblast, mesoderm and epiblast of gastrulating chick embryos have some HNK-1-related proteins in common, while others are unique to specific tissues. Attempts have been made to identify these proteins using Western blots and antibodies known to recognize HNK-1-related molecules, but none of the antibodies used identify the bands unique to any of the tissues studied. We conclude that these proteins may be novel members of the HNK-1/L2 family, and that they may have a role in cell interactions during early development.
通过单克隆抗体 (HNK-1) 识别的人类 NK 和 K 细胞的分化抗原。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Abo,T;Balch,CM
通讯作者：
Balch,CM