Downregulation of ST3GAL5 is associated with muscle invasion, high grade and a poor prognosis in patients with bladder cancer
Downregulation of ST3GAL5 is associated with muscle invasion, high grade and a poor prognosis in patients with bladder cancer
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ST3GAL5 下调与膀胱癌患者的肌肉侵袭、高级别和不良预后相关
DOI：
10.3892/ol.2020.11597
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发表时间：
2020-07-01
期刊：
影响因子：
2.9
通讯作者：
Wang, Qin-Zhang
中科院分区：
文献类型：
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作者：
Ouyang, Song;Liu, Ji-Hong;Wang, Qin-Zhang
在膀胱癌 (BC) 患者中，ST3 β-半乳糖苷 α-2,3-唾液酸转移酶 5 (ST3GAL5) 表达与临床结果之间的关联，特别是在肌肉侵袭性疾病、高肿瘤分级和预后方面，仍不清楚。在本研究中，使用各种公共生物信息学数据库分析了 BC 患者中 ST3GAL5 的表达及其与临床结果的关联。还使用 Oncomine 数据库、癌症基因组图谱和基因表达综合数据库的数据评估了 BC 和健康膀胱组织之间 ST3GAL5 表达的差异。还分析了肌肉侵袭性 BC（MIBC）和非肌肉侵袭性 BC（NMIBC）以及高级别和低级别 BC 之间 ST3GAL5 表达的差异。此外，通过 Oncomine、Gene Expression Profiling Interactive Analysis 2 和 UALCAN 数据库之间的交集，鉴定出 BC 患者中与 ST3GAL5 呈阳性共表达的基因。使用 R 软件包进行基因本体论、京都基因和基因组百科全书的富集分析、反应组途径富集分析和基因概念网络。还进行了基因集富集分析，以评估 BC 中 ST3GAL5 高表达和低表达影响的信号通路。结果表明，与正常膀胱组织相比，BC组织中ST3GAL5的表达量显着降低（P
In patients with bladder cancer (BC), the association between ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (ST3GAL5) expression and clinical outcomes, particularly regarding muscle-invasive disease, high tumor grade and prognosis, remain unknown. In the present study, the expression of ST3GAL5 and its association with clinical outcomes in patients with BC was analyzed using various public bioinformatics databases. The difference in ST3GAL5 expression between BC and healthy bladder tissues was also evaluated using data from the Oncomine database, The Cancer Genome Atlas and Gene Expression Omnibus database. The differences in ST3GAL5 expression between muscle invasive BC (MIBC) and non-muscle invasive BC (NMIBC), and high- and low-grade BC were also analyzed. Furthermore, genes that were positively co-expressed with ST3GAL5 in patients with BC were identified from the intersection between the Oncomine, Gene Expression Profiling Interactive Analysis 2 and UALCAN databases. Enrichment analysis by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, Reactome pathway enrichment analyses and a gene-concept network was performed using R package. Gene set enrichment analysis was also performed to assess the signaling pathways influenced by the high and low expression of ST3GAL5 in BC. The results indicated that ST3GAL5 expression was significantly lower in BC tissues compared with normal bladder tissues (P