RAPID ASSAY OF FOREIGN GENE-EXPRESSION IN LEAF-DISKS TRANSFORMED BY AGROBACTERIUM-TUMEFACIENS - ROLE OF T-DNA BORDERS IN THE TRANSFER PROCESS
RAPID ASSAY OF FOREIGN GENE-EXPRESSION IN LEAF-DISKS TRANSFORMED BY AGROBACTERIUM-TUMEFACIENS - ROLE OF T-DNA BORDERS IN THE TRANSFER PROCESS
复制标题
DOI：
10.1073/pnas.83.12.4428
复制
发表时间：
1986-06-01
影响因子：
11.1
通讯作者：
KLEE, HJ
中科院分区：
文献类型：
--
作者：
HORSCH, RB;KLEE, HJ
我们已经开发了一种敏感的叶盘转化程序，用于研究根癌农杆菌介导的植物细胞转化过程中的早期和/或瞬时T-DNA表达。利用这个系统，我们已经研究了T-DNA边界序列的功能，T-DNA基因的早期表达和这些基因在感染细胞中的稳定整合。T-DNA右边界的缺失似乎允许从肿瘤诱导（Ti）质粒转移到T-DNA基因，但大大降低了它们稳定整合的频率。已经构建了一个双元载体，以允许检查T-DNA边界功能的反式Ti质粒。在这种情况下，单个T-DNA边界对于T-DNA基因的早期表达是必需的，并且足以在任何方向上稳定整合。
We have developed a sensitive leaf disc transformation procedure for studying early and/or transient T-DNA expression during Agrobacterium tumefaciens-mediated transformation of plant cells. Using this system, we have examined the function of T-DNA border sequences on the early expression of T-DNA genes and on the stable integration of those genes in infected cells. Deletion of the right border from the T-DNA appears to permit transfer to T-DNA genes from the tumor-inducing (Ti) plasmid but greatly reduces the frequency of their stable integration. A binary vector has been constructed to permit examination of T-DNA border function in trans to the Ti plasmid. In this situation, a single T-DNA border is necessary for early expression of T-DNA genes and is sufficient for stable integration in any orientation.