Two new endophytic species enrich the Coniochaeta endophytica / C. prunicola clade: Coniochaeta lutea sp. nov. and C. palaoa sp. nov.
Two new endophytic species enrich the Coniochaeta endophytica / C. prunicola clade: Coniochaeta lutea sp. nov. and C. palaoa sp. nov.
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DOI：
10.35535/pfsyst-2021-0006
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发表时间：
2021-07-01
影响因子：
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通讯作者：
Inderbitzin, Patrik
中科院分区：
文献类型：
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作者：
Arnold, A. Elizabeth;Harrington, Alison H.;Inderbitzin, Patrik
Coniochaeta （Coniochaetaceae，子囊菌门）是一个多样化的属，包括具有内生生活方式的未描述物种的惊人丰富，特别是在温带和北方植物和地衣中。这些内生菌通常代表未被描述的物种，可以澄清以前分类的真菌分支内的进化关系和特征进化。在此，我们扩展了地理、分类和寄主采样，这些采样呈现在先前对含有内生孢子毛藻（Coniochaeta endophytica）的进化枝的分析中，内生孢子毛藻是最近被描述的一种来自北美的内生真菌；和C. prunicola，与南非核桃树的坏死有关。我们对亚利桑那大学（ARIZ） Robert L. Gilbertson真菌学植物标本馆内的内生菌菌株的多位点分析和元数据检查(1)将内生菌的地理范围扩展到比以前认识到的更广泛的美国范围；(2)表明在美国北卡罗来纳州和密歇根州，原型prunicola菌（CBS 120875）与本地宿主内生菌形成了一个支持良好的支系；(3)揭示了C. prunicola （CBS 121445）的前副型与来自北卡罗莱纳和俄罗斯的内生菌形成了一个不同的分支，在形态上与本文考虑的其他分类群不同，本文将其描述为Coniochaeta lutea；(4)描述了一个新种Coniochaeta palaoa，这里被鉴定为北卡罗莱纳州高地和山前地区多个植物谱系的内生菌。CBS 120875和CBS 121445分离为严格意义的C. prunicola和C. lutea与之前描述的这些分离株之间的基因组差异一致，并且四个物种（C. endophytica， C. prunicola， C. palaoa和C. lutea）之间的形态和功能差异强调了本文所述的系统发育关系。本文讨论了特定位点的分辨能力，以及对Coniochaeta和内生菌C. / C. prunicola分支的宿主和地理范围的新看法。
Coniochaeta (Coniochaetaceae, Ascomycota) is a diverse genus that includes a striking richness of undescribed species with endophytic lifestyles, especially in temperate and boreal plants and lichens. These endophytes frequently represent undescribed species that can clarify evolutionary relationships and trait evolution within clades of previously classified fungi. Here we extend the geographic, taxonomic, and host sampling presented in a previous analysis of the clade containing Coniochaeta endophytica, a recently described species occurring as an endophyte from North America; and C. prunicola, associated with necroses of stonefruit trees in South Africa. Our multi-locus analysis and examination of metadata for endophyte strains housed in the Robert L. Gilbertson Mycological Herbarium at the University of Arizona (ARIZ) (1) expands the geographic range of C. endophytica across a wider range of the USA than recognized previously; (2) shows that the ex-type of C. prunicola (CBS 120875) forms a well-supported clade with endophytes of native hosts in North Carolina and Michigan, USA; (3) reveals that the ex-paratype for C. prunicola (CBS 121445) forms a distinct clade with endophytes from North Carolina and Russia, is distinct morphologically from the other taxa considered here, and is described herein as Coniochaeta lutea; and (4) describes a new species, Coniochaeta palaoa, here identified as an endophyte of multiple plant lineages in the highlands and piedmont of North Carolina. Separation of CBS 120875 and CBS 121445 into C. prunicola sensu stricto and C. lutea is consistent with previously described genomic differences between these isolates, and morphological and functional differences among the four species (C. endophytica, C. prunicola, C. palaoa, and C. lutea) underscore the phylogenetic relationships described here. The resolving power of particular loci and the emerging perspective on the host- and geographic range of Coniochaeta and the C. endophytica / C. prunicola clade are discussed.