CALCIUM ACCUMULATION AND NEURONAL DAMAGE IN THE RAT HIPPOCAMPUS FOLLOWING CEREBRAL-ISCHEMIA
CALCIUM ACCUMULATION AND NEURONAL DAMAGE IN THE RAT HIPPOCAMPUS FOLLOWING CEREBRAL-ISCHEMIA
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DOI：
10.1038/jcbfm.1987.13
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发表时间：
1987-02-01
影响因子：
6.3
通讯作者：
WIELOCH, T
中科院分区：
文献类型：
--
作者：
DESHPANDE, JK;SIESJO, BK;WIELOCH, T
本研究旨在探讨短暂性脑缺血后钙蓄积与神经元坏死的关系。在大鼠前脑缺血10min后--这段时间导致CA1锥体细胞的可复制损伤--在不同的再循环时间进行背侧海马区钙浓度的测定和细胞体坏死的形态标志的评估。组织钙离子浓度在缺血期结束时与对照组无差异，再灌流后3、6、12、24 h无明显变化。48h后钙离子含量显著升高，72h后进一步升高，恢复早期仅见散在的坏死神经元。48h后，12个大脑半球中只有2个大脑半球每张切片显示坏死细胞超过25个。72h后神经元死亡更为明显，表明海马上区钙的净积聚先于CA1锥体细胞的明显坏死。结果表明，这些细胞延迟性死亡的一个主要事件是膜功能障碍和钙循环的增加。
The present study was undertaken to correlate calcium accumulation with the development of neuronal necrosis following transient ischemia. After 10 min of forebrain ischemia in the rat-a period that leads to reproducible damage of CA1 pyramidal cells-determination of calcium concentration and evaluation of morphological signs of cell body necrosis in the dorsal hippocampus were performed at various recirculation times. Tissue calcium concentration was not different from control at the end of ischemic period and did not change after 3, 6, 12, or 24 h of recirculation. However, after 48 h, calcium content increased significantly, with a further increase being seen after 72 h. At early recovery periods, only scattered necrotic neurons were observed. After 48 h, only 2 of 12 hemispheres showed more than 25 necrotic cells per section. More conspicuous neuronal death was observed after 72 h. The results thus demonstrate that net accumulation of calcium in regio superior of the hippocampus precedes marked necrosis of CA 1 pyramidal cells. The results suggest that one primary event in the delayed death of these cells is membrane dysfunction with increased calcium cycling.