The eubacterium Ectothiorhodospira halophila is negatively phototactic, with a wavelength dependence that fits the absorption spectrum of the photoactive yellow protein
The eubacterium Ectothiorhodospira halophila is negatively phototactic, with a wavelength dependence that fits the absorption spectrum of the photoactive yellow protein
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DOI：
10.1128/jb.175.10.3096-3104.1993
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发表时间：
1993-05
影响因子：
3.2
通讯作者：
W. W. Sprenger-W.;W. Hoff;J. Armitage;K. Hellingwerf
中科院分区：
文献类型：
--
作者：
W. W. Sprenger-W.;W. Hoff;J. Armitage;K. Hellingwerf
能动、嗜碱且极嗜盐的紫硫细菌 Ectothiorhodospira halophila 具有正避光性。这种反应导致细菌在光斑中积累（E. Hustede, M. Liebergesell, and H. G. Schlegel, Photochem. Photobiol. 50:809-815, 1989 ；D. E. McRee, J. A. Tainer, T. E. Meyer, J. Van Beeumen, M. A. Cusanovich, and E. D. Getzoff, Proc. Natl. Acad.美国科学。 86：6533-6537，1989；另外，这项工作）。在这项研究中，我们证明了嗜盐桉也具有负趋光性。对自由游动的细菌和厌氧细胞悬浮液中光色边界形成的细胞分布模式的视频分析揭示了对强烈（但非破坏性）蓝光的排斥反应的存在。在存在饱和背景光合光的情况下，蓝光强度的增加会引起方向转换，而强蓝光的减少则会抑制这些反转。据我们所知，这是自由游动的真细菌对光真正的驱避反应的第一份报告，因为大肠杆菌和鼠伤寒沙门氏菌的蓝光反应（B. L. Taylor and D. E. Koshland, Jr., J. Bacteriol. 123:557-569, 1975）需要极高的光强度，不太可能是一个感觉过程。这种负光响应的波长依赖性是用窄带通干涉滤光片确定的。它显示出与来自嗜盐大肠杆菌的光敏黄色蛋白的吸收光谱相似。
The motile, alkalophilic, and extremely halophilic purple sulfur bacterium Ectothiorhodospira halophila is positively photophobotactic. This response results in the accumulation of bacteria in light spots (E. Hustede, M. Liebergesell, and H. G. Schlegel, Photochem. Photobiol. 50:809-815, 1989; D. E. McRee, J. A. Tainer, T. E. Meyer, J. Van Beeumen, M. A. Cusanovich, and E. D. Getzoff, Proc. Natl. Acad. Sci. USA 86:6533-6537, 1989; also, this work). In this study, we demonstrated that E. halophila is also negatively phototactic. Video analysis of free-swimming bacteria and the formation of cell distribution patterns as a result of light-color boundaries in an anaerobic suspension of cells revealed the existence of a repellent response toward intense (but nondamaging) blue light. In the presence of saturating background photosynthetic light, an increase in the intensity of blue light induced directional switches, whereas a decrease in intense blue light gave rise to suppression of these reversals. To our knowledge, this is the first report of a true repellent response to light in a free-swimming eubacterium, since the blue light response in Escherichia coli and Salmonella typhimurium (B. L. Taylor and D. E. Koshland, Jr., J. Bacteriol. 123:557-569, 1975), which requires an extremely high light intensity, is unlikely to be a sensory process. The wavelength dependence of this negative photoresponse was determined with narrow band pass interference filters. It showed similarity to the absorption spectrum of the photoactive yellow protein from E. halophila.