Control of juvenile hormone biosynthesis. Evidence for phosphorylation of the 3-hydroxy-3-methylglutaryl coenzyme A reductase of insect corpus allatum.
Control of juvenile hormone biosynthesis. Evidence for phosphorylation of the 3-hydroxy-3-methylglutaryl coenzyme A reductase of insect corpus allatum.
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DOI：
10.1016/s0021-9258(19)68126-3
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发表时间：
1982-02
期刊：
影响因子：
--
通讯作者：
D. Monger;J. H. Law
中科院分区：
文献类型：
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作者：
D. Monger;J. H. Law
在从烟草天蛾成虫的咽侧体制备的匀浆上清液（10，000 × g）中测量了保幼激素生物合成的关键酶3-羟基-3-甲基戊二酰辅酶A还原酶的活性。酶活性被抑制80%的50 mM氟化钠，一种已知的磷蛋白磷酸酶抑制剂，如果在腺体摘除和所有随后的组织检查过程中存在。在用4 mM MgCl 2和2 mM ATP预孵育的匀浆中，还原酶活性也显著降低（20-30%）。这些结果提供了证据，在昆虫的还原酶经历类似的哺乳动物肝脏的还原酶发生的磷酸化和去磷酸化。如果是这样的话，这将提供一种快速调节保幼激素合成的方法。
The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a key enzyme of juvenile hormone biosynthesis, have been measured in the supernatants of homogenates (10,000 x g) prepared from the corpora allata of the adult tobacco hornworm moth, Manduca sexta. Enzyme activity was inhibited 80% by 50 mM NaF, a known phosphoprotein phosphatase inhibitor, if present during extirpation of the glands and all subsequent workup of the tissue. Reductase activity was also significantly decreased (20-30%) in homogenates preincubated with 4 mM MgCl2 and 2 mM ATP. These results provide evidence that reductase in the insect undergoes phosphorylation and dephosphorylation similar to that occurring with reductase of mammalian liver. If so, this would provide a rapid method for modulating juvenile hormone synthesis.