In vitro response of monocyte-derived macrophages to a decellularized pericardial biomaterial
In vitro response of monocyte-derived macrophages to a decellularized pericardial biomaterial
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DOI：
10.1002/jbm.a.32554
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发表时间：
2010-04-01
影响因子：
4.9
通讯作者：
Lee, J. Michael
中科院分区：
文献类型：
--
作者：
Ariganello, Marianne B.;Labow, Rosalind S.;Lee, J. Michael
脱细胞组织衍生的心脏瓣膜都是来源于天然支架的生物材料。这些类型的植入物越来越受欢迎，尽管它们在体内的性能仍然知之甚少，有时甚至是灾难性的。显然，在安全使用这些生物材料之前，需要更好的了解。本研究以人单核细胞源性巨噬细胞（MDM）对脱细胞牛心包（DBP）的反应为模型，预测这些材料油内植入的生物学性能。将在组织培养聚苯乙烯（TCPS）上分化14天的人单核细胞胰蛋白酶化后，再接种于DBP、TCPS和聚二甲基硅氧烷（PDMS）上48小时。DBP上的MDMs细胞内和细胞外酯酶活性低于MDMs油TCPS和PDMS，酸性磷酸酶活性低于油TCPS。在形态学上，MDMs油型DBP分布较少，多核较少，片状足较少。综上所述，这些数据首次证明了MDM对完整的天然细胞外基质的反应，表明与两个对照表面相比，这些细胞对这种天然支架的所有改变的、可能减少的异物反应都发生了反应。这种体外MDM细胞模型可以为预测和阐明组织源性生物材料的生物学性能提供一种新的方法，从而指导更合理地设计用于组织再生目的的生物材料。(C) 2009 Wiley期刊公司[J] .中国生物医学工程学报，2016,31 (1):1 - 4
Decellularized tissue-derived heart valves are all example of biomaterials derived from natural scaffolds. These types of implants are increasing in popularity although their in vivo performance is still only poorly understood and has, at times, been catastrophic. It is apparent that better understanding is required before these biomaterials can be used safely. In this study, the human monocyte-derived macrophage (MDM) response to decellularized bovine pericardium (DBP) Was used as a model to predict the biological performance of these materials oil implaotation. Human monocytes differentiated on tissue Culture polystyrene (TCPS) for 14 days were trypsinized and reseeded onto DBP, TCPS, and polydimethylsiloxane (PDMS) for 48 h. The MDMs on DBP contained less intracellular and extracellular esterase activity compared with MDMs oil TCPS and PDMS, as well as less acid phosphatase activity than oil TCPS. As well, morphologically, MDMs oil DBP were less spread, less multinucleated and did not display many lamellipodia. Taken together, these data represent the first evidence of the MDM response to intact, native extracellular matrix, demonstrating that these cells reacted with all altered, possibly reduced foreign body response oil this natural scaffold compared with the two control surfaces. This in vitro MDM cell model may provide a novel method for predicting and elucidating the biological performance Of tissue-derived biomaterials, thereby directing a more rational design of biomaterials for tissue regeneration purposes. (C) 2009 Wiley Periodicals, Inc. J Biomed Matcr Res 93A: 280-288, 2010