An improved and simplified radioisotopic assay for the determination of free and esterified carnitine.
An improved and simplified radioisotopic assay for the determination of free and esterified carnitine.
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一种改进和简化的放射性同位素测定法，用于测定游离肉碱和酯化肉碱。
DOI：
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发表时间：
1976
影响因子：
6.5
通讯作者：
D. W. Foster
中科院分区：
文献类型：
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作者：
J. McGarry;D. W. Foster
Cederblad和Lindstedt（Clin. Chim. Acta. 37：235-543，1972），并由Bohmer等人（Clin. Chim. Acta. 57：55-61，1974）已经被改进和简化。因此，该测定在广泛的肉毒碱浓度范围内产生线性响应，而不需要过量的标记乙酰辅酶A。此外，它将测量非常少量的肉毒碱，即使在存在过量的酰基肉毒碱。该方法允许快速测定小体积血浆（50穆尔就足够了）中的游离和酯化肉毒碱，而不需要预先对样品进行脱蛋白处理。
The radioisotopic assay for carnitine first described by Cederblad and Lindstedt (Clin. Chim. Acta. 37:235-543, 1972) and modified by Bohmer et al (Clin. Chim. Acta. 57:55-61, 1974) has been improved and simplified. As a result, the assay yields a linear response over a wide range of carnitine concentrations without the need for excessive amounts of labeled acetyl-CoA. In addition, it will measure very small quantities of carnitine even in the presence of excess acylcarnitine. The method allows rapid determination of free and esterified carnitine in small volumes of plasma (50 mul is sufficient) without the need for prior deproteinization of the samples.