Effects of Rich-Polyphenols Extract of Dendrobium loddigesii on Anti-Diabetic, Anti-Inflammatory, Anti-Oxidant, and Gut Microbiota Modulation in db/db Mice
Effects of Rich-Polyphenols Extract of Dendrobium loddigesii on Anti-Diabetic, Anti-Inflammatory, Anti-Oxidant, and Gut Microbiota Modulation in db/db Mice
复制标题
富含铁皮石斛多酚提取物对 db/db 小鼠抗糖尿病、抗炎、抗氧化和肠道菌群调节的影响
DOI：
10.3390/molecules23123245
复制
发表时间：
2018-12-01
期刊：
影响因子：
4.6
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
Li, Xue-Wen;Chen, Hui-Ping;Wang, Jun
石斛是一种具有抗糖尿病作用的传统中草药，含有多种联苯和菲。针对石斛多酚类化合物抗糖尿病作用知之甚少的现状，采用富含多酚类物质的石斛提取物(DJP)对糖尿病db/db小鼠进行治疗，并对其血清生化指标和组织形态进行了评价。采用酶联免疫吸附试验、免疫组织化学及16S rRNA基因高通量测序等方法，对糖尿病大鼠的氧化应激指标、肿瘤坏死因子α、白介素6及肠道微生物区系进行检测，以探讨其抗糖尿病的作用机制。结果表明，DJP对小鼠血糖、体重、低密度脂蛋白胆固醇(LDL-C)水平有降低作用，对胰岛素(INS)水平有升高作用。改善小鼠脂肪肝和糖尿病肾病。DJP具有降低丙二醛(MDA)水平，提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽(GSH)含量的抗氧化作用。丹参冲剂具有降低IL-6、肿瘤坏死因子-α表达的抗炎作用。治疗后，小鼠肠道菌群平衡得到改善，类杆菌与非杆菌的比值增加，普氏杆菌/阿克曼氏菌的相对丰度增加，S24-7/里克氏杆菌/大肠杆菌的相对丰度降低。肠道微生物区系的功能预测表明，碳水化合物代谢或脂肪代谢的微生物组成发生了变化。本研究首次揭示DJP能改善糖尿病小鼠的症状和并发症，这可能是由于DJP减轻炎症和氧化应激，改善肠道菌群平衡的作用。
Dendrobium is a traditional Chinese herb with anti-diabetic effects and has diverse bibenzyls as well as phenanthrenes. Little is known about Dendrobium polyphenols anti-diabetic activities, so, a rich-polyphenols extract of D. loddigesii (DJP) was used for treatment of diabetic db/db mice; the serum biochemical index and tissue appearance were evaluated. In order to gain an insight into the anti-diabetic mechanism, the oxidative stress index, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and gut microbiota modulation were determined by ELISA, immunohistochemistry or high throughput sequencing 16S rRNA gene. The results revealed that DJP had the effects to decrease the blood glucose, body weight, low density lipoprotein cholesterol (LDL-C) levels and increase insulin (INS) level in the mice. DJP improved the mice fatty liver and diabetic nephropathy. DJP showed the anti-oxidative abilities to reduce the malondialdehyde (MDA) level and increase the contents of superoxide dismutase (SOD), catalase (CAT) as well as glutathione (GSH). DJP exerted the anti-inflammatory effects of decreasing expression of IL-6 and TNF-α. After treatment of DJP, the intestinal flora balance of the mice was ameliorated, increasing Bacteroidetes to Firmicutes ratios as well as the relative abundance of Prevotella/Akkermansia and reducing the relative abundance of S24-7/Rikenella/Escherichia coli. The function’s prediction of gut microbiota indicated that the microbial compositions involved carbohydrate metabolism or lipid metabolism were changed. This study revealed for the first time that DJP improves the mice symptoms of diabetes and complications, which might be due to the effects that DJP induced the decrease of inflammation as well as oxidative stress and improvement of intestinal flora balance.