Comprehensive scanning of somatic mitochondrial DNA mutations in breast cancer.
Comprehensive scanning of somatic mitochondrial DNA mutations in breast cancer.
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DOI：
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发表时间：
2002-02
期刊：
影响因子：
11.2
通讯作者：
D. Tan;Ren-kui Bai;L. Wong
中科院分区：
文献类型：
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作者：
D. Tan;Ren-kui Bai;L. Wong
为了研究线粒体DNA（mtDNA）在肿瘤发生中的作用，采用时间温度梯度凝胶电泳方法分析乳腺癌中的体细胞mtDNA突变。使用 32 对重叠引物扩增 19 个肿瘤样本和来自同一患者的配对正常组织中的整个线粒体基因组。对正常线粒体 DNA 和肿瘤线粒体 DNA 之间显示不同带型的 DNA 片段进行测序，以鉴定突变。 19 个肿瘤中有 14 个（74%）显示出至少一种体细胞 mtDNA 突变。发现了 27 个体细胞突变，其中 22 个发生在 D 环区域。这项研究代表了乳腺癌中最全面的 mtDNA 突变分析。
To investigate the role of mitochondrial DNA (mtDNA) in tumorigenesis, a temporal temperature gradient gel electrophoresis method was used to analyze the somatic mtDNA mutations in breast cancer. The entire mitochondrial genomes in 19 tumor samples and paired normal tissues from the same patients were amplified using 32 pairs of overlapping primers. DNA fragments showing different banding patterns between normal and tumor mtDNA were sequenced to identify the mutations. Fourteen of the 19 tumors (74%) displayed at least one somatic mtDNA mutation. Twenty-seven somatic mutations were found, and 22 of them occurred in the D loop region. This study represents the most comprehensive mtDNA mutational analysis in breast cancer.