Immunomodulation of Labeo rohita juveniles due to dietary gelatinized and non-gelatinized starch
Immunomodulation of Labeo rohita juveniles due to dietary gelatinized and non-gelatinized starch
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DOI：
10.1016/j.fsi.2006.11.008
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发表时间：
2007-08-01
影响因子：
4.7
通讯作者：
Kumar, Shivendra
中科院分区：
文献类型：
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作者：
Kumar, Vikas;Sahu, N. P.;Kumar, Shivendra
进行了 60 天的实验来研究日粮糊化 (G) 和非糊化 (NG) 淀粉对 Labeo rohita 幼鱼免疫调节的影响。 234 只幼鱼（平均重量 2.53 +/- 0.04）随机分布在 6 个处理组中，每组重复 3 个。制备了六种含有 NG 和 G 玉米淀粉的半纯化日粮，每种饲料有 6 个含量水平（0、20、40、60、80、100），即 T-1（100% NG、0% G 淀粉）、T-2（80% NG、20% G 淀粉）、T-3（60% NG、40% G 淀粉）、T-4（40% NG、 60% G 淀粉）、T-5（20% NG、80% G 淀粉）和 T-6（0% NG、100% G 淀粉）。经过 60 天的喂养期后，幼鱼受到嗜水气单胞菌的攻击，以研究其因喂养 G 和 NG 淀粉而产生的免疫调节作用。由于细菌攻击，红细胞和血红蛋白含量显着降低（P < 0.05），但膳食淀粉（G/NG淀粉）对此没有影响。饲料中的 G:NG 淀粉比例对攻击前后的白细胞总数有显着影响。随着饮食中G淀粉水平的增加，白细胞计数也随之增加。在攻击前和攻击后，T6 组（100% G 淀粉）的白蛋白/球蛋白（A/G）比率最高，T1 组（100% NG 淀粉）最低，其次是 T2 组。在攻击前和攻击后，NBT、溶菌酶活性、总蛋白和球蛋白含量在 T2 组（80% NG、20% G 淀粉）中最高。用嗜水气单胞菌攻击后，T2组的存活率最高，而T6组的存活率最低。最终发现，高水平的 G 淀粉对 Labeo rohita 幼鱼具有免疫抑制作用，80:20 的 NG:G 淀粉比例似乎是促进 L. rohita 幼鱼生长和保护免疫力的最佳选择。 (C) 2006 Elsevier Ltd. 保留所有权利。
A 60 days experiment was conducted to study the effect of dietary gelatinized (G) and non-gelatinized (NG) starch on immunomodulation of Labeo rohita juveniles. Two hundred and thirty four juveniles (av. wt. 2.53 +/- 0.04) were randomly distributed in six treatment groups with each of three replicates. Six semi-purified diets containing NG and G corn starch, each at six levels of inclusion (0, 20, 40, 60, 80, 100) were prepared viz., T-1 (100% NG, 0% G starch), T-2 (80% NG, 20% G starch), T-3 (60% NG, 40% G starch), T-4 (40% NG, 60% G starch), T-5 (20% NG, 80% G starch) and T-6 (0% NG, 100% G starch). After a feeding period of 60 days, the juveniles were challenged with Aeromonas hydrophila to study their immunomodulation due to feeding of G and NG starch. RBC and haemoglobin content were significantly (P < 0.05) reduced due to bacterial challenge, but dietary starch (G/NG starch) had no effect on it. G:NG starch ratio in the feed had significant effect on total leukocyte count during pre- and post-challenge periods. The leukocyte count concomitantly increased with the increased level of G starch in the diet. Highest albumin/globulin (A/G) ratio was recorded in T6 group (100% G starch) and lowest in T1 group (100% NG starch) group followed by T2 group both in pre- and post-challenge periods. NBT, lysozyme activity, total protein and globulin content were highest in T2 group (80% NG, 20% G starch) both in pre- and post-challenge periods. After challenge with A. hydrophila, the highest survival was recorded in T2 group, whereas lowest survival was recorded in T6 group. Conclusively high level of G starch was found to be immunosuppressive in Labeo rohita juveniles and NG:G starch ratio of 80:20 seems to be optimum for promoting growth and protecting immunity in L. rohita juveniles. (C) 2006 Elsevier Ltd. All rights reserved.