Apoptosis‐specific protein (ASP 45 kDa) is distinct from human Apg5, the homologue of the yeast autophagic gene apg5
Apoptosis‐specific protein (ASP 45 kDa) is distinct from human Apg5, the homologue of the yeast autophagic gene apg5
复制标题
DOI：
10.1016/s0014-5793(02)03497-x
复制
发表时间：
2002-11
期刊：
影响因子：
3.5
通讯作者：
H. Yung;Luzheng Xue;A. Tolkovsky
中科院分区：
文献类型：
--
作者：
H. Yung;Luzheng Xue;A. Tolkovsky
我们研究了凋亡特异性蛋白p45ASP和人类Apg5是否相同的蛋白。与p45ASP一样，myc-hApg5与c-Jun抗体交叉反应，在HeLa细胞中，约50%的myc-hApg5与Triton x -100不溶部分结合。然而，可溶性myc-hApg5在staurosporine或TNFα/环己亚胺诱导的细胞凋亡过程中被降解，而可溶性p45ASP的表达稳定。此外，myc-hApg5的降解被caspase抑制剂Boc-Asp(OMe)FMK阻断，同时p45ASP的表达被消除。此外，myc-hApg5 （~ 32 kDa）从未假设p45ASP （45 kDa）的大小。因此，p45ASP和人类Apg5可能是不同的蛋白质，尽管它们确实有一些共同的特征。
We have examined whether the apoptosis-specific protein p45ASP and human Apg5 are identical proteins. Like p45ASP, myc-hApg5 cross-reacted with a c-Jun antibody and ∼50% of myc-hApg5 was bound to a Triton X-100-insoluble fraction in HeLa cells. However, soluble myc-hApg5 was degraded during apoptosis induced by staurosporine or TNFα/cycloheximide whilst expression of soluble p45ASP was stabilised. Furthermore, myc-hApg5 degradation was blocked by the caspase inhibitor Boc-Asp(OMe)FMK whilst p45ASP expression was eliminated. Moreover, myc-hApg5 (∼32 kDa) never assumed the size of p45ASP (45 kDa). It is therefore likely that p45ASP and human Apg5 are distinct proteins although they do share some common characteristics.