Antigens recognized by IgG derived from tumor-infiltrating B lymphocytes in human lung cancer.
Antigens recognized by IgG derived from tumor-infiltrating B lymphocytes in human lung cancer.
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发表时间：
2006-09
影响因子：
2
通讯作者：
M. Yasuda;Makiko Mizukami;T. Hanagiri;Y. Shigematsu;Takashi Fukuyama;Y. Nagata;T. So;Y. Ichiki;M. Sugaya;M. Takenoyama;K. Sugio;K. Yasumoto
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作者：
M. Yasuda;Makiko Mizukami;T. Hanagiri;Y. Shigematsu;Takashi Fukuyama;Y. Nagata;T. So;Y. Ichiki;M. Sugaya;M. Takenoyama;K. Sugio;K. Yasumoto
肺癌组织常被B淋巴细胞浸润，但尚不清楚这些浸润是肿瘤特异性免疫反应还是非特异性反应。材料与方法利用移植新鲜人肺癌的严重联合免疫缺陷（SCID）小鼠模型对重组cDNA表达文库（SEREX）进行血清学分析。在这里，一组抗原识别肿瘤浸润B淋巴细胞（TIB）在人肺癌的特征。结果改进的SEREX分析在肺大细胞癌中鉴定出22种不同的抗原。序列分析和real - time-PCR分析显示，55%的分离抗原在肿瘤组织中过表达，9%的抗原发生突变。结论在异种移植SCID小鼠模型中可以检测到肺癌患者TIB的体液免疫反应，我们的修饰对肿瘤抗原的鉴定具有较高的敏感性和特异性。
BACKGROUND Lung cancer tissues are often infiltrated by B lymphocytes, but it is not clear whether these infiltrations represent tumor-specific immune response or a nonspecific reaction. MATERIALS AND METHODS The serological analysis of recombinant cDNA expression libraries (SEREX) were previously modified using a severe combined immunodeficient (SCID) mice model engrafted with fresh human lung cancer. Here, a panel of antigens recognized by tumor-infiltrating B lymphocytes (TIB) in human lung cancer were characterized. RESULTS The modified SEREX analysis identified 22 distinct antigens in a large cell carcinoma of the lung. Sequence analysis and real time-PCR analysis showed that 55% of isolated antigens were overexpressed in tumor tissues and 9% had mutation. CONCLUSION The results of this study indicate that the humoral immune response of TIB in lung cancer patients can be detected in the xenotransplanted SCID mouse model and our modification shows high sensitivity and specificity for identification of tumor antigens.