Broad-spectrum chemicals block ROS detoxification to prevent plant fungal invasion.
Broad-spectrum chemicals block ROS detoxification to prevent plant fungal invasion.
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DOI：
10.1016/j.cub.2022.07.022
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发表时间：
2022-09-26
期刊：
影响因子：
--
通讯作者：
Liang W
中科院分区：
文献类型：
--
作者：
Yang Q;Yang J;Wang Y;Du J;Zhang J;Luisi BF;Liang W
植物病害对粮食安全造成巨大影响，受到全球关注。虽然施用农用化学品是目前控制植物病害的常用方法，但这些化合物日益增长的耐药性和脱靶效应的影响构成了重大挑战。目前迫切需要确定与致病性有关的毒力机制，并开发针对这些过程的新化学品。一种这样的毒力机制是在病原体攻击时由宿主植物产生的活性氧（ROS）的解毒。因此，ROS解毒机制可能作为预防植物病害的药物靶标，但很少有抗ROS清除药物被开发出来。在这里，我们表明，在模型系统灰葡萄孢分泌的细胞色素c-过氧化物酶，BcCcp 1去除植物产生的H2 O2，促进病原体入侵。过氧化物酶的分泌是由一个Tom 1样蛋白，BcTol 1，通过物理相互作用。我们发现，BcTol 1在不同的水平进行调节，以提高在感染早期分泌的BcCcp 1。BcTol 1或BcCcp 1的失活导致B的毒力显著降低。灰色区域。我们确定了两个BcTol 1靶向小分子，不仅防止B。灰霉病菌入侵，而且还具有有效的活性，对广泛的植物真菌病原体没有可检测的影响主机。这些发现揭示了真菌中ROS解毒的保守机制，并提供了一类潜在的杀菌剂来控制多种植物病害。本文所述的方法对相关领域的进一步药物发现具有广泛的意义。
Plant diseases cause a huge impact on food security and are of global concern. While application of agrochemicals is a common approach in the control of plant diseases currently, growing drug resistance and the impact of off-target effects of these compounds pose major challenges. The identification of pathogenicity-related virulence mechanisms and development of new chemicals that target these processes are urgently needed. One such virulence mechanism is the detoxification of reactive oxygen species (ROS) generated by host plants upon attack by pathogens. The machinery of ROS detoxification might therefore serve as a drug target for preventing plant diseases, but few anti-ROS-scavenging drugs have been developed. Here, we show that in the model system Botrytis cinerea secretion of the cytochrome c-peroxidase, BcCcp1 removes plant-produced H2O2 and promotes pathogen invasion. The peroxidase secretion is modulated by a Tom1-like protein, BcTol1, through physical interaction. We show that BcTol1 is regulated at different levels to enhance the secretion of BcCcp1 during the early infection stage. Inactivation of either BcTol1 or BcCcp1 leads to dramatically reduced virulence of B. cinerea. We identify two BcTol1-targeting small molecules that not only prevent B. cinerea invasion but also have effective activity against a wide range of plant fungal pathogens without detectable effect on the hosts. These findings reveal a conserved mechanism of ROS detoxification in fungi and provide a class of potential fungicides to control diverse plant diseases. The approach described here has wide implications for further drug discovery in related fields.
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植物病原体灰葡萄孢中赖氨酸乙酰化的全蛋白质组分析
影响因子：
4.6
作者：
Lv B;Yang Q;Li D;Liang W;Song L
通讯作者：
Song L
影响因子：
4.9
作者：
Hollomon, DW;Butters, JA;Hall, L
通讯作者：
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DOI：
10.3390/plants10112303
发表时间：
2021-10-26
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Kokhmetova A;Rsaliyev A;Malysheva A;Atishova M;Kumarbayeva M;Keishilov Z
通讯作者：
Keishilov Z
影响因子：
6.7
作者：
Huang K;Czymmek KJ;Caplan JL;Sweigard JA;Donofrio NM
通讯作者：
Donofrio NM
影响因子：
3.5
作者：
Gronover, CS;Kasulke, D;Tudzynski, B
通讯作者：
Tudzynski, B