SPECIES-DIFFERENCES IN THE DISTRIBUTION OF SUBSTANCE-P AND TYROSINE-HYDROXYLASE IMMUNOREACTIVITY IN THE OLFACTORY-BULB
SPECIES-DIFFERENCES IN THE DISTRIBUTION OF SUBSTANCE-P AND TYROSINE-HYDROXYLASE IMMUNOREACTIVITY IN THE OLFACTORY-BULB
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DOI：
10.1002/cne.902520206
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发表时间：
1986-10-08
影响因子：
2.5
通讯作者：
BAKER, H
中科院分区：
文献类型：
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作者：
BAKER, H
这些研究记录了许多哺乳动物物种（包括小鼠、大鼠、豚鼠、兔、猫和两种仓鼠（中国仓鼠和叙利亚仓鼠））中枢神经系统区域内肽物质P和儿茶酚胺合成酶酪氨酸羟化酶（TH）分布的种属差异。在两种仓鼠的主嗅球（MOB）中观察到含P物质的神经元胞体，但在其他物种的MOB中未观察到。然而，在副嗅球（AOB），神经元染色观察到所有物种，除了小鼠。染色胞体的数量及其强度各不相同，因此标记在大鼠中最显著，其次是家兔、豚鼠、猫和仓鼠，依次递减。小鼠未显示核周染色。AOB染色胞体存在于内部颗粒细胞层中，树突状突起穿过内部丛状层分支到二尖瓣细胞层内。P物质染色的神经元在MOB的分布也不同的两个仓鼠染色。在叙利亚仓鼠，神经元主要是肾小球。在中国仓鼠的肾小球区和外网状层（EPL）的表面都发现了标记的胞体。两种物种中大多数P物质标记的神经元的平均最长直径大于10 μ m，表明它们是簇状细胞。中国仓鼠的EPL中的那些是最大的（17 μ M）。在MOB内P物质阳性轴突和终末的分布也观察到种属差异。标记主要分布在叙利亚仓鼠的内部颗粒细胞层和中国仓鼠的内部丛状层。除叙利亚仓鼠外，种属间酪氨酸羟化酶染色相似。在后者的物种，一个额外的大人口的神经元被发现在外部丛状层。在所有其他物种中，TH染色的神经元被发现分散在整个MOB，偶尔AOB，但在EPL中并不多。尽管大多数TH神经元大于10 μ m，但在所有物种中，主要在肾小球层中观察到较小TH细胞群，这表明大多数TH标记的神经元是簇状细胞，但一些可能是肾小球周围细胞。总之，这些数据表明，无论是P物质是存在于仓鼠的MOB，但没有发现在小鼠，大鼠，豚鼠，兔，或猫的主要嗅球的肾小球神经元，或P物质的水平或在其处理前体存在种属差异。在任何一种情况下，通过使用相同的固定和染色程序，肽可以定位在一些物种中，而不是其他物种，而酪氨酸羟化酶染色是相似的，在所有物种的检查。
These studies document species differences in the distribution of the peptide substance P and the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) within a central nervous system region of a number of mammalian species including the mouse, rat, guinea pig, rabbit, cat, and two species of hamster (Chinese and Syrian). Substance P-containing neuronal perikarya were observed in the main olfactory bulb (MOB) of both species of the hamster, but not in the MOB of the other species examined. In the accessory olfactory bulb (AOB), however, neuronal staining was observed in all species except the mouse. The number of stained somata and their intensity varied such that label was most prominent in the rat followed in the decreasing order by the rabbit, guinea pig, cat, and hamster. The mouse displayed no perikaryal staining. Stained somata in AOB were found in the internal granule cell layer with dendrite processes ramifying through the internal plexiform layer to arborize within the mitral cell layer. The distribution of substance P-stained neurons in the MOb also differed between the two hamster stains. In the Syrian hamster, neurons were primarily juxtaglomerular. In the Chinese hamster, labeled perikarya were found in both the juxtaglomerular region and within the superficial aspect of the external plexiform layer (EPL). The mean longest diameter of the majority of substance P-labeled neurons in both species was greater than 10 .mu.m, suggesting that they were tufted cells. Those in the EPL of the Chinese hamster were the largest (17 .mu.M). Species differences also were observed in the distribution of substance P-positive axons and terminals within the MOB. Label was distributed primarily in the internal granule cell layer of the Syrian hamster and the internal plexiform layer of the Chinese hamster. Tyrosine hydroxylase staining was similar among species with the exception of the Syrian hamster. In the latter species, an additional large population of neurons was found within the external plexiform layer. In all other species, TH-stained neurons were found scattered throughout the MOB and occasionally the AOB but were not numerous in the EPL. Although most TH neurons were larger than 10 .mu.m, in all species a population of smaller TH cells was observed primarily in the glomerular layer, suggesting that most neurons labeled with TH are tufted cells but that some may be periglomerular cells. In conclusion these data suggest that either substance P is present in the MOB of hamsters but is not found in juxtaglomerular neurons of the main olfactory bulb of the mouse, rat, guinea pig, rabbit, or cat, or that species differences exist in the level of substance P or in its processing from precursors. In either case, by using the same fixation and staining procedures, the peptides can be localized in some and not other species, whereas tyrosine hydroxylase staining is similar in all species examined.