Effects of insulin on vasoconstriction induced by thromboxane A2 in porcine coronary artery.
Effects of insulin on vasoconstriction induced by thromboxane A2 in porcine coronary artery.
复制标题
胰岛素对血栓素 A2 诱导的猪冠状动脉血管收缩的影响。
DOI：
10.1161/01.cir.81.5.1654
复制
发表时间：
1990
期刊：
影响因子：
37.8
通讯作者：
Tsuneaki Sugimoto
中科院分区：
文献类型：
--
作者：
Atsuko Yanagisawa;Hideki Ito;Tsuneaki Sugimoto
为阐明胰岛素在冠状动脉张力调控中的作用，本实验观察了胰岛素对血栓素A2（TXA 2）诱发的猪离体冠状动脉收缩的影响。将猪近端冠状动脉的环形制备物悬浮在充满台氏溶液的Magnus装置中，在37 ℃下，用100%O2通气，并测量收缩的等长张力。胰岛素本身既不引起收缩也不引起舒张。胰岛素对20 mM K+、去甲肾上腺素、组胺和5-羟色胺诱发的冠状动脉收缩无显著影响;然而，用生理浓度的胰岛素预孵育120分钟，（30-300 μ单位/ml）显著增强STA 2（10（-11）至10（-7）M）诱发的冠状动脉收缩，一种稳定的TXA 2类似物，已知作用于TXA 2/前列腺素H2受体（在300 μ单位/ml胰岛素存在下，10（-7）M STA 2时为对照的141.4 +/-10.9%; p <0.01）。胰岛素对STA 2诱导的收缩的增强作用受细胞外葡萄糖或镁离子浓度的影响。胰岛素的增强作用仅在葡萄糖浓度为100-300 mg/dl和镁浓度为0.5-1.5 mM时观察到。因此，怀疑胰岛素通过依赖于细胞外葡萄糖和Mg 2+的过程增强TXA 2诱导的收缩。
To elucidate the role of insulin in the control of coronary artery tone, its effects on porcine coronary artery contraction evoked by thromboxane A2 (TXA2) were studied in vitro. Ring preparations of porcine proximal coronary artery were suspended in a Magnus apparatus filled with Tyrode's solution at 37 degrees C and aerated with 100% O2, and the isometric tension of the contractions was measured. Insulin itself caused neither contraction nor relaxation. Insulin had no significant effect on the coronary artery contractions evoked by 20 mM K+, norepinephrine, histamine, and serotonin; however, 120 minutes of preincubation with a physiological concentration of insulin (30-300 muunits/ml) significantly accentuated coronary artery contractions evoked by STA2 (10(-11) to 10(-7) M), a stable analogue of TXA2 that is known to act on TXA2/prostaglandin H2 receptors (141.4 +/- 10.9% of the control at 10(-7) M STA2 in the presence of 300 muunits/ml insulin; p less than 0.01). The enhancing effects of insulin on the STA2-induced contractions were affected by extracellular glucose or magnesium ion concentrations. The enhancing effects of insulin were observed only at the glucose concentrations of 100-300 mg/dl and magnesium concentrations of 0.5-1.5 mM. Therefore, insulin was suspected of enhancing TXA2-induced contraction through a process that depends on extracellular glucose and Mg2+.
影响因子：
3.2
作者：
KRIEGER, DR;LANDSBERG, L
通讯作者：
LANDSBERG, L
胰岛素增加 BC3H-1 肌细胞的膜和胞质蛋白激酶 C 活性。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cooper,DR;Konda,TS;Standaert,ML;Davis,JS;Pollet,RJ;Farese,RV
通讯作者：
Farese,RV