Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi
Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi
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DOI：
10.1007/s13205-017-1015-z
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发表时间：
2017-10-30
期刊：
影响因子：
2.8
通讯作者：
Wang, Cong
中科院分区：
文献类型：
--
作者：
Guo, Lei;Wang, Cong
采用Plackett-Burman设计和Box-Behnken设计等统计方法，对海洋来源黄曲霉HN4-13菌株产生抗哈维氏弧菌活性物质的发酵条件进行了优化。获得了哈维氏弧菌活性物质产量最高的关键发酵值为：X-1(蛋白调)=0.3%，X-2(KCl)=0.25%，X-3(接种量)=4.5%。对哈维氏弧菌抑菌圈直径预测值为23.39 mm，实测值为23.71+/-0.98 mm，增幅为62.3%。生物测定引导的分离得到了两个化合物，其结构被鉴定为Questin(1)和大黄素(2)。奎斯汀对哈维氏弧菌具有与链霉素相同的抗菌活性(MIC为31.25 mU g/mL)。这是首次报道Questin作为一种潜在的抗菌剂来对抗水生病原菌V.harveyi。
Statistical methodologies, including Plackett-Burman design and Box-Behnken design, were employed to optimize the fermentation conditions for the production of active substances against aquatic pathogen Vibrio harveyi by marine-derived Aspergillus flavipes strain HN4-13. The optimal crucial fermentation values for maximum production of active substances against V. harveyi were obtained as follows: X-1 (peptone) = 0.3%, X-2 (KCl) = 0.25%, and X-3 (inoculum size) = 4.5%. The predicted diameter of inhibitory zone against V. harveyi was 23.39 mm, and the practical value reached 23.71 +/- 0.98 mm with a 62.3% increase. Bioassay-guided fractionation resulted in the acquisition of two compounds whose structures were identified as questin (1) and emodin (2). Questin exhibited the same antibacterial activity against V. harveyi as streptomycin (MIC 31.25 mu g/mL). This is the first time to report questin as a potential antibacterial agent against aquatic pathogen V. harveyi.