BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation
BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation
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BDNF 由 TPO 刺激的巨核细胞系产生，调节自分泌增殖
DOI：
10.1016/j.bbrc.2012.09.093
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发表时间：
2012
影响因子：
3.1
通讯作者：
Takanori Moriyama
中科院分区：
文献类型：
--
作者：
Shogo Tamura;Ayumi Nagasawa;Yuya Masuda;Tetsuya Tsunematsu;Koji Hayasaka;Kazuhiko Matsuno;Chikara Shimizu;Yukio Ozaki;Takanori Moriyama
虽然人类血小板在活化时释放内源性脑源性神经营养因子（BDNF），但先前关于MEG-01（一种巨核细胞系）的报告没有发现BDNF产生的痕迹，并且血小板BDNF的病理生理功能仍然难以捉摸。在本研究中，我们证明了MEG-01在TPO存在下产生BDNF，这有助于增强细胞增殖。我们在体外的研究结果表明，BDNF调节MEG-01增殖的自分泌方式，我们认为，BDNF可能是一个生理性的巨核祖细胞的自分泌调节。
While human platelets release endogenous brain-derived neurotrophic factor (BDNF) upon activation, a previous report on MEG-01, a megakaryocytic cell line, found no trace of BDNF production, and the pathophysiological function of platelet BDNF has remained elusive. In the present study, we demonstrate that MEG-01 produces BDNF in the presence of TPO and that this serves to potentiate cell proliferation. Our in vitro findings suggest that BDNF regulates MEG-01 proliferation in an autocrine manner, and we suggest that BDNF may be a physiological autocrine regulator of megakaryocyte progenitors.