Quantum dot bioconjugates for ultrasensitive nonisotopic detection
Quantum dot bioconjugates for ultrasensitive nonisotopic detection
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DOI：
10.1126/science.281.5385.2016
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发表时间：
1998-09-25
期刊：
影响因子：
56.9
通讯作者：
Nie, SM
中科院分区：
文献类型：
--
作者：
Chan, WCW;Nie, SM
高发光半导体量子点(硫化锌修饰的硒化镉)已被共价偶联到生物分子上，用于超灵敏的生物检测。与罗丹明等有机染料相比，这类发光标签的亮度是罗丹明的20倍，抗光漂白的稳定性是100倍，光谱线宽是罗丹明的三分之一。这些纳米结合物是水溶性和生物相容性的，标记了转铁蛋白的量子点在培养的HeLa细胞中经历了受体介导的内吞作用，标记了免疫分子的量子点识别了特定的抗体或抗原。
Highly luminescent semiconductor quantum dots (zinc sulfide-capped cadmium selenide) have been covalently coupled to biomolecules for use in ultrasensitive biological detection. In comparison with organic dyes such as rhodamine, this class of luminescent labels is 20 times as bright, 100 times as stable against photobleaching, and one-third as wide in spectral linewidth. These nanometer-sized conjugates are water-soluble and biocompatible, Quantum dots that were labeled with the protein transferrin underwent receptor-mediated endocytosis in cultured HeLa cells, and those dots that were labeled with immunomolecules recognized specific antibodies or antigens.