MADS box genes expressed in developing inflorescences of rice and sorghum
MADS box genes expressed in developing inflorescences of rice and sorghum
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DOI：
10.1007/s004380050364
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发表时间：
1997-02-20
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影响因子：
--
通讯作者：
Pe, ME
中科院分区：
文献类型：
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作者：
Greco, R;Stagi, L;Pe, ME
为了阐明控制谷类植物花形态发生的复杂遗传系统，我们从发育中的花序的cDNA文库中分离了两个虱子和两个高粱MADS盒基因。水稻无性系OsMADS24和OsMADS45与拟南芥AGL2和AGL4 MADS box基因有很高的同源性，在花分生组织、所有原基和成熟花器官中均有表达。嗨，哦，在发育中的籽粒中也发现了表达水平。高粱克隆SbMADS1也与AGL2和AGL4同源：表达分析和定位数据表明它是OsMAADS24的直系同源。另一个高粱MADS盒基因SbMADS2的表达模式表明，它可能作为分生组织识别基因发挥作用，就像拟南芥中的AP1一样，它与拟南芥有相当大的同源性。这四个基因已被定位在水稻RFLP遗传图谱上：根据谷类之间的共性对结果进行了讨论。
With the aim of elucidating the complex genetic system controlling flower morphogenesis in cereals, we have characterized two lice and two sorghum MADS box genes isolated from cDNA libraries made from developing inflorescences. The rice clones OsMADS24 and OsMADS45, which share high homology with the Arabidopsis AGL2 and AGL4 MADS box genes, are expressed in the floral meristem, in all the primordia, and in mature floral organs. Hi,oh expression levels have also been found in developing kernels. The sorghum clone SbMADS1 is also homologous to AGL2 and AGL4: expression analysis and mapping data suggest that it is the ortholog of OsMAADS24. The pattern of expression of SbMADS2, the other sorghum MADS box gene, suggests that it may play a role as a meristem identity gene, as does AP1 in Arabidopsis, to which it shows considerable homology. The four genes have been mapped on a rice RFLP genetic map: the results are discussed in terms of synteny among cereals.