Serological Evidence of Hepatitis E Virus (HEV) Infection Among Ruminant Farmworkers: A Retrospective Study from Malaysia.
Serological Evidence of Hepatitis E Virus (HEV) Infection Among Ruminant Farmworkers: A Retrospective Study from Malaysia.
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反刍动物农场工人戊型肝炎病毒 (HEV) 感染的血清学证据：来自马来西亚的回顾性研究
DOI：
10.2147/idr.s367394
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发表时间：
2022
影响因子：
3.9
通讯作者：
Lin, Yulan
中科院分区：
文献类型：
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作者：
Wong, Li Ping;Tay, Sun Tee;Chua, Kek Heng;Goh, Xiang Ting;Alias, Haridah;Zheng, Zizheng;Zhao, Qinjian;Wu, Ting;Xia, Ningshao;Hu, Zhijian;Lin, Yulan
由于缺乏有关马来西亚戊型肝炎病毒（HEV）感染的数据，本研究旨在确定马来西亚反刍动物农场工人中HEV的血清阳性率。方法采集8个养殖场87名养殖工人的血清标本。采用北京万泰生物制药企业有限公司生产的万泰HEV-IgG和HEV-IgM ELISA试剂盒，通过酶联免疫吸附试验（ELISA）检测所有血清样本的抗-HEVIgG和抗-HEVIgM。Ltd.，Beijing，China.结果调查对象为6个养牛场、1个养羊场和1个养羊场的农民工。只有一个农场实行零放牧，其他农场实行轮牧。在87名农场工人中，男性占83.9%，几乎一半（47.1%）年龄在20-35岁之间。按种族划分，绝大多数是马来人。大多数农场工人都有良好的卫生习惯;在处理农场动物后清洗或更换衣服和淋浴是常见的。未检测到抗-HEVIgM和IgG（95%可信区间（CI）：0，0.0415）。结论该农场工人无戊型肝炎暴露史，不存在职业暴露的危险。我们的研究结果表明，在穆斯林占多数的国家，反刍动物农场工人中HEV感染的血清阳性率为零。良好的农场管理，卫生习惯和没有接触猪相关的污染可能有助于没有或最小的人畜共患病的风险戊型肝炎病毒在本研究中调查的农场工人。
Background As scant data are available about Hepatitis E virus (HEV) infection in Malaysia, this study aimed to determine the seroprevalence of HEV amongst ruminant farmworkers in Malaysia. Methods A total of 87 farmworkers provided serum samples, which were collected from eight farms. All serum samples were tested for anti-HEV IgG and anti-HEV IgM by an enzyme-linked immunosorbent assay (ELISA) using the Wantai HEV-IgG and HEV-IgM ELISA kits from Beijing Wantai Biological Pharmacy Enterprise Co., Ltd, Beijing, China. Results Farmworkers from six cattle farms, one sheep farm and one goat farm were investigated in this study. Only one farm practices zero-grazing, with the rest using rotational grazing. Of the 87 farmworkers, males comprised 83.9%, and almost half (47.1%) were aged 20–35 years old. By ethnic group, the vast majority were Malay. Most of the farmworkers have good hygiene practices; washing or changing their clothes and showering after dealing with farm animals were common. None of the farmworker serum samples had anti-HEV IgM and IgG detected (95% confidence interval (CI): 0, 0.0415). Conclusion The finding suggests that the farmworkers had no previous exposure to Hepatitis E, and were not at risk of occupational exposure to HEV infection. Our findings suggest that a zero seroprevalence of HEV infection among ruminant farmworkers in the Muslim majority country. Good farm management, hygiene practices and the absence of contact with swine-related contamination might have contributed to the no or minimal zoonotic risks of HEV amongst farmworkers surveyed in this study.