Biosynthesis of volatiles by the myxobacterium Myxococcus xanthus
Biosynthesis of volatiles by the myxobacterium Myxococcus xanthus
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DOI：
10.1002/cbic.200300813
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发表时间：
2004-06-07
期刊：
影响因子：
3.2
通讯作者：
Schulz, S
中科院分区：
文献类型：
--
作者：
Dickschat, JS;Wenzel, SC;Schulz, S
利用闭环汽提装置（CLSA）对黄色粘球菌（Myxococcusxanthus）细胞培养物的挥发性成分进行了分离和GC-MS分析，鉴定并合成了两个新的天然产物：（S）-9-甲基癸-3-醇（（S）-1）和9-甲基癸-3-酮（2），以及其它脂肪酮、醇和萜烯类化合物。在用野生型菌株DK 1622和两种突变菌株JD 300和KD 11017进行的饲养实验中检查了两种主要组分（S）-12的含量，所述突变菌株JD 300和KD 11017在从亮氨酸到异戊酰-SCoA的降解途径中受损。异戊酰-SCoA用作起始剂，随后用两个丙二酸酯单元进行链延长。随后使用丙二酸甲酯和脱羧得到（S）-1和2。此外，突变菌株可使用3，3_二甲基丙烯酸（DMAA）形成异戊酰- SCoA，这证实了最近关于检测到从HMGCoA产生异戊酰-SCoA的甲羟戊酸途径的新品种的数据。
The volatiles emitted from cell cultures of myxobacterium Myxococcus xanthus were collected by use of a closed-loop stripping apparatus (CLSA) and analyzed by GC-MS. Two new natural products, (S)-9-methyldecan-3-ol ((S)-1) and 9-methyldecan-3-one (2), were identified and synthesized, together with other aliphatic ketones and alcohols, and terpenes, Biosynthesis, of the two main components (S)-1 2 was examined in feeding experiments carried out with the wild-type strain DK1622 and two mutant strains JD300 and KD11017, which are impaired in the degradation pathway from leucine to isovaleryl-SCoA. Isovaleryl-SCoA is used as a starter, followed by chain elongation with two malonate units. Subsequent use of methyl malonate and decarboxylation leads to (S)-1 and 2. Furthermore, 3,3-dimethylacrylic acid (DMAA) can be used by the mutant strain to form isovaleryl- SCoA, which corroborates, recent data on the detection of a novel variety of the mevalonate pathway giving rise to isovaleryl-SCoA from HMGCoA.