SEMI-ROLLED LEAF1 Encodes a Putative Glycosylphosphatidylinositol-Anchored Protein and Modulates Rice Leaf Rolling by Regulating the Formation of Bulliform Cells
SEMI-ROLLED LEAF1 Encodes a Putative Glycosylphosphatidylinositol-Anchored Protein and Modulates Rice Leaf Rolling by Regulating the Formation of Bulliform Cells
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SEMI-ROLLED LEAF1 编码一种假定的糖基磷脂酰肌醇锚定蛋白，并通过调节泡状细胞的形成来调节水稻叶卷
DOI：
10.1104/pp.112.199968
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发表时间：
2012-08-01
期刊：
影响因子：
7.4
通讯作者：
Xue, Hong-Wei
中科院分区：
文献类型：
--
作者：
Xiang, Jing-Jing;Zhang, Guang-Heng;Xue, Hong-Wei
卷叶是水稻育种中一项重要的农艺性状，适度的卷叶可以保持叶片的直立，减少叶片间的遮蔽，从而提高光合效率和籽粒产量。虽然已经发现了一些卷叶突变体，并分离出一些控制卷叶的基因，但卷叶的分子机制仍有待阐明。本文报道了一个半滚叶1 （semi - rollleaf1, SRL1）基因的分离和鉴定。突变体SRL1 -1（点突变）和SRL1 -2（转移DNA插入）由于近轴细胞层的球状细胞数量增加而表现出正面卷曲的叶片，这可以通过SRL1的互补表达来挽救。SRL1在多种组织中表达，在球状细胞中表达水平较低。SRL1蛋白位于质膜上，预计是一种假定的糖基磷脂酰肌醇锚定蛋白。此外，通过激光捕获显微解剖分析srl1-1中野生型表皮细胞和可能成为球泡状细胞的细胞的基因表达谱，发现在球泡状细胞形成过程中增加的空泡H+- atp酶（亚基A、B、C和D）和H+-焦磷酸酶的基因表达在srl1-1中上调。这些结果提供了水稻叶片细胞向球泡状细胞转变的转录谱，表明SRL1通过负调控液泡H+- atp酶亚基和H+-焦磷酸酶编码基因的表达，抑制球泡状细胞的形成，从而调控叶片滚动，这将有助于了解叶片滚动的调控机制。
Leaf rolling is an important agronomic trait in rice (Oryza sativa) breeding and moderate leaf rolling maintains the erectness of leaves and minimizes shadowing between leaves, leading to improved photosynthetic efficiency and grain yields. Although a few rolled-leaf mutants have been identified and some genes controlling leaf rolling have been isolated, the molecular mechanisms of leaf rolling still need to be elucidated. Here we report the isolation and characterization of SEMI-ROLLED LEAF1 (SRL1), a gene involved in the regulation of leaf rolling. Mutants srl1-1 (point mutation) and srl1-2 (transferred DNA insertion) exhibit adaxially rolled leaves due to the increased numbers of bulliform cells at the adaxial cell layers, which could be rescued by complementary expression of SRL1. SRL1 is expressed in various tissues and is expressed at low levels in bulliform cells. SRL1 protein is located at the plasma membrane and predicted to be a putative glycosylphosphatidylinositol-anchored protein. Moreover, analysis of the gene expression profile of cells that will become epidermal cells in wild type but probably bulliform cells in srl1-1 by laser-captured microdissection revealed that the expression of genes encoding vacuolar H+-ATPase (subunits A, B, C, and D) and H+-pyrophosphatase, which are increased during the formation of bulliform cells, were up-regulated in srl1-1. These results provide the transcript profile of rice leaf cells that will become bulliform cells and demonstrate that SRL1 regulates leaf rolling through inhibiting the formation of bulliform cells by negatively regulating the expression of genes encoding vacuolar H+-ATPase subunits and H+-pyrophosphatase, which will help to understand the mechanism regulating leaf rolling.