Structural organization and unusual codon usage in the DNA polymerase gene from herpes simplex virus type 1.
Structural organization and unusual codon usage in the DNA polymerase gene from herpes simplex virus type 1.
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1 型单纯疱疹病毒 DNA 聚合酶基因的结构组织和异常密码子使用。
DOI：
10.1089/dna.1986.5.281
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Mount,DW
中科院分区：
文献类型：
--
作者：
Hall,JD;Gibbs,JS;Coen,DM;Mount,DW
我们分析了单纯疱疹病毒1型（HSV-1）的DNA聚合酶的蛋白质和核酸序列，以了解这种酶的表达和可能的结构。广泛的氨基酸序列之间的相似性和爱泼斯坦巴尔病毒DNA聚合酶的报道。我们描述了这些蛋白质之间可能的结构相似性和使用这些相似性来定义聚合酶内的结构和功能域。碱基组成和密码子使用的分析表明，几个基因从HSV-1，包括DNA聚合酶，表现出强烈的偏好鸟嘌呤或胞嘧啶在第三个密码子的位置。这种偏好可能是由于病毒的高鸟嘌呤+胞嘧啶含量，并产生与宿主细胞不同的高度限制的密码子使用。病毒表达的不寻常密码子使用的后果包括广泛的mRNA二级结构的可能性。
We have analyzed the protein and nucleic acid sequences of the DNA polymerase from herpes simplex virus type 1 (HSV-1) to provide insight into the expression and possible structure of this enzyme. Extensive similarity between the amino acid sequence and that of the Epstein Barr virus DNA polymerase is reported. We describe probable structural similarities between these proteins and the use of these similarities to define structural and functional domains within the polymerase. Analysis of base composition and codon usage reveals that several genes from HSV-1, including DNA polymerase, exhibit a strong preference for guanine or cytosine at the third codon position. This preference may result from the high guanine + cytosine content of the virus and produces a highly restricted codon usage, different from that of the host cell. Consequences of the unusual codon usage for viral expression include the potential for extensive mRNA secondary structure.