Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles
Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles
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DOI：
10.1016/j.micinf.2006.12.017
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发表时间：
2007-03-01
影响因子：
5.8
通讯作者：
Bachmann, Martin F.
中科院分区：
文献类型：
--
作者：
Gatto, Dominique;Bauer, Monika;Bachmann, Martin F.
边缘区 (MZ) B 细胞在功能、表型和定位特性方面与滤泡 (FO) B 细胞不同。目前尚不清楚来自 MZ 区室的 B 细胞是否也具有独特或偏向的 BCR 特异性，仅识别有限数量的保守抗原结构。为了解决边缘区 B 细胞免疫反应的复杂性，我们比较了用两种不同的病毒样颗粒 (VLP) 免疫诱导的小鼠 MZ 和 FO 13 细胞的抗体库。从分选的 VLP 特异性 MZ 和 FO B 细胞中分离的抗体序列在重链 V、D 和 J 基因片段使用方面相似。对来自 MZ 和 FO B 细胞的抗体 CDR3 区域的序列分析也显示 N 核苷酸添加或 CDR3 长度没有一致的差异。相反，与来自 FO B 细胞的抗体相比，来自 MZ B 细胞的抗体 CDR 区域的体细胞超突变减少。这些结果表明 MZ B 细胞对 VLP 的反应具有克隆型异质性，并表明 MZ B 细胞区室能够产生可变且多样化的抗体反应。 (c) 2007 年 Elsevier Masson SAS。版权所有。
Marginal zone (MZ) B cells differ from follicular (FO) B cells in their functional, phenotypic and localization properties. It is still unclear whether B cells from the MZ compartment also have distinct or biased BCR specificities, recognizing only a limited number of conserved antigenic structures. To address the complexity of the immune response mounted by marginal zone B cells, we compared the antibody repertoire of murine MZ and FO 13 cells induced by immunization with two different virus-like particles (VLPs). Antibody sequences isolated from sorted VLP-specific MZ and FO B cells were similar in heavy chain V, D and J gene segment usage. Sequence analysis of CDR3 regions of antibodies from MZ and FO B cells also revealed no consistent difference in N nucleotide additions or CDR3 length. In contrast, somatic hypermutations were reduced in CDR regions of antibodies from MZ B cells compared to those from FO B cells. These results indicate that the response of MZ B cells to VLPs is clonotypically heterogeneous and suggest that the MZ B cell compartment is capable of generating variable and diverse antibody responses. (c) 2007 Elsevier Masson SAS. All rights reserved.