Authenticity control of game meat products - A single method to detect and quantify adulteration of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon) by real-time PCR
Authenticity control of game meat products - A single method to detect and quantify adulteration of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon) by real-time PCR
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DOI：
10.1016/j.foodchem.2014.08.048
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发表时间：
2015-03-01
期刊：
影响因子：
8.8
通讯作者：
Cichna-Markl, Margit
中科院分区：
文献类型：
--
作者：
Druml, Barbara;Grandits, Stephanie;Cichna-Markl, Margit
本文提出了一种单一的实时PCR检测方法，可以测定肉制品中鹿的含量（休闲鹿（达玛达玛），红鹿（马鹿）和西卡（日本鹿）的总和），以检测食品掺假。PCR检测未显示与野味肉制品中可能含有的20种动物物种和43种植物物种的交叉反应性。对于休闲鹿和红鹿，定量限为0.5%，对于西卡，定量限为0.1%。肉制品中鹿的含量是通过将鹿PCR检测获得的浓度与扩增哺乳动物和家禽DNA的参考系统获得的浓度相关联来确定的。对含有猪肉的二元肉混合物、在猪肉中含有等量的休闲鹿、红鹿和西卡鹿的肉混合物以及模型野味香肠的分析表明，定量方法是非常准确的（通常是系统误差
This contribution presents a single real-time PCR assay allowing the determination of the deer content (the sum of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon)) in meat products to detect food adulteration. The PCR assay does not show cross-reactivity with 20 animal species and 43 botanical species potentially contained in game meat products. The limit of quantification is 0.5% for fallow deer and red deer and 0.1% for sika deer. The deer content in meat products is determined by relating the concentration obtained with the deer PCR assay to that obtained with a reference system which amplifies mammals and poultry DNA. The analysis of binary meat mixtures with pork, a meat mixture containing equal amounts of fallow deer, red deer and sika deer in pork and a model game sausage showed that the quantification approach is very accurate (systematic error generally