Chloride channel blockers decrease intracellular pH in cultured renal epithelial LLC-PK1 cells
Chloride channel blockers decrease intracellular pH in cultured renal epithelial LLC-PK1 cells
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DOI：
10.1111/j.1476-5381.1996.tb15422.x
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发表时间：
1996-06-01
影响因子：
7.3
通讯作者：
Dudley, AJ
中科院分区：
文献类型：
--
作者：
Brown, CDA;Dudley, AJ
在LLC-PK 1细胞的肾上皮单层中检查氯离子通道阻断剂对细胞内pH（pH（i））的影响。发现加入100 μ M 5-硝基-2-（3-苯基丙基氨基）-苯甲酸酯（NPPB）、尼氟灭酸、氟灭酸和二苯胺-2-羧酸酯（DPC）时细胞内酸化显著，但加入4，4 '-二异硫氰酸基二苯乙烯-2-2'-二磺酸（DIDS）时细胞内酸化不显著。这些试剂对pH（i）的影响是剂量依赖性的，表观K-0.5值分别为：尼氟灭酸、氟灭酸和DPC为16.7+/-0.3 μ M，34.2+/-0.9 μ M和740+/-13 μ M。结果表明，在通常用于阻断通道活性的浓度下，这些氯离子通道阻断剂对pH（i）具有深远的影响。
The effects of chloride channel blockers upon intracellular pH (pH(i)) were examined in renal epithelial monolayers of LLC-PK1 cells. A significant intracellular acidification was found with addition of 100 mu M 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), niflumic acid, flufenamate and diphenylamine-2-carboxylate (DPC) but not with 4,4'-diisothiocyanatostilbene-2-2'disulphonic acid (DIDS). The effects of these agents upon pH(i) was dose-dependent with apparent K-0.5 values of: 16.7+/-0.3 mu M, 34.2+/-0.9 mu M and 740+/-13 mu M for niflumic acid, flufenamate and DPC respectively. The results indicate that at concentrations commonly used to block channel activity these chloride channel blockers have profound effects upon pH(i).