Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles
Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles
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DOI：
10.1016/j.ecoenv.2015.06.025
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发表时间：
2015-10-01
影响因子：
6.8
通讯作者：
Azizi, Morteza
中科院分区：
文献类型：
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作者：
Amooaghaie, Rayhaneh;Saeri, Mohammad Reza;Azizi, Morteza
尽管纳米技术领域具有发展潜力，但纳米颗粒对环境和人类健康可能产生的影响令人担忧。本研究采用“绿色法”和“化学法”合成了银纳米粒子。在湿化学法中，以硼氢化钠、柠檬酸钠和硝酸银为原料。在绿色合成法中，采用黑种草叶提取物作为还原剂和封端剂还原银银。此外，两种合成的AgNPs的毒性反应进行了监测，对小鼠的骨生成干细胞，以及种子萌发和幼苗生长的六种不同的植物（黑麦草，小麦，豆类和普通的野豌豆，生菜和油菜）。在这两种合成方法中，无色的反应混合物变成棕色，紫外-可见光谱证实了银纳米颗粒的存在。扫描电子显微镜（SEM）观察显示银纳米尺寸的微晶和傅立叶变换红外光谱（FTIR）的优势表明在合成过程中的不同官能团的作用。KIT检测显示，使用黑孜然提取物合成的绿色AgNPs中小鼠成骨干细胞的细胞活力比化学AgNPs更高。绿色银纳米颗粒对6种植物种子萌发、根长和芽长的IC 50值均高于化学银纳米颗粒。这些结果表明，绿色合成的银纳米粒子的细胞毒性和植物毒性显着低于湿化学合成的。本研究为利用白刺叶片进行生态友好型栽培提供了一种经济、简便、高效的技术。sativa用于合成AgNP，并证实了绿色AgNP比化学合成的AgNP更安全。(C)© 2015 Elsevier Inc版权所有。
Despite the development potential in the field of nanotechnology, there is a concern about possible effects of nanoparticles on the environment and human health. In this study, silver nanoparticles (AgNPs) were synthesized by 'green' and 'chemical' methods. In the wet-chemistry method, sodium borohydrate, sodium citrate and silver nitrate were used as raw materials. Leaf extract of Nigella sativa was used as reducing as well as capping agent to reduce silver nitrate in the green synthesis method. In addition, toxic responses of both synthesized AgNPs were monitored on bone-building stem cells of mice as well as seed germination and seedling growth of six different plants (Lolium, wheat, bean and common vetch, lettuce and canola). In both synthesis methods, the colorless reaction mixtures turned brown and UV-visible spectra confirmed the presence of silver nanoparticles. Scanning electron microscope (SEM) observations revealed the predominance of silver nanosized crystallites and fourier transform infra-red spectroscopy (FTIR) indicated the role of different functional groups in the synthetic process. KIT assay showed cell viability of bone-building stem cells of mice was further in the green AgNPs synthesized using black cumin extract than chemical AgNPs. IC50 (inhibitory concentrations) values for seed germination, root and shoot length for 6 plants in green AgNPs exposures were higher than the chemical AgNPs. These results suggest that cytotoxicity and phytotoxicity of the green synthesized AgNPs were significantly less than wet-chemistry synthesized ones. This study indicated an economical, simple and efficient ecofriendly technique using leaves of N. sativa for synthesis of AgNPs and confirmed that green AgNPs are safer than chemically-synthesized AgNPs. (C) 2015 Elsevier Inc All rights reserved.