Whole-Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample.
Whole-Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample.
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DOI：
10.1002/anie.201507047
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发表时间：
2015-11-16
期刊：
影响因子：
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通讯作者：
Weitz DA
中科院分区：
文献类型：
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作者：
Han HS;Cantalupo PG;Rotem A;Cockrell SK;Carbonnaux M;Pipas JM;Weitz DA
宏基因组研究表明，自然界中仅存在一小部分病毒已被识别和研究。任何病毒样本中存在的许多非特异性基因组阻碍了未知病毒基因组的表征。在这里，我们报告了一个集成了基于液滴的微流体和计算分析的新平台，该平台能够从复杂的混合病毒样本中纯化任何单一病毒物种并检索完整的基因组序列。使用该平台，我们检索了掺入废水中的 5243 bp dsDNA 病毒的基因组序列，序列覆盖率 > 96%，同一性 > 99.8%。该平台在病毒发现方面具有巨大的潜力，因为它可以对以前未描述的病毒以及已知的病毒进行富集和测序。
Metagenomic studies suggest that only a small fraction of viruses existing in nature have been identified and studied. Characterization of unknown viral genomes is hindered by many non-specific genomes populating any virus sample. Here, we report a new platform integrating drop-based microfluidics and computational analysis that enables purification of any single viral species from a complex, mixed virus sample and the retrieval of complete genome sequences. Using this platform, we retrieve the genome sequence of a 5243 bp dsDNA virus that was spiked into wastewater with > 96% sequence coverage and > 99.8% identity. This platform holds great potential for virus discovery as it allows enrichment and sequencing of previously undescribed viruses as well as known viruses.