A new MSPQC for rapid growth and detection of Mycobacterium tuberculosis
A new MSPQC for rapid growth and detection of Mycobacterium tuberculosis
复制标题
用于结核分枝杆菌快速生长和检测的新型 MSPQC
DOI：
10.1016/j.bios.2008.04.018
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发表时间：
2008-11-15
影响因子：
12.6
通讯作者：
Cui, Xiaoying
中科院分区：
文献类型：
--
作者：
Ren, Jiali;He, Fengjiao;Cui, Xiaoying
世界上许多结核病(TB)造成的死亡都是由于诊断错误或诊断晚了。为此，世界卫生组织(WHO)呼吁开发更好、更便宜的结核病检测方法。本文研制了一种新型的多通道串联压电石英晶体(MSPQC)传感器系统，用于结核分枝杆菌的快速生长和检测。它是一种自动连续监测系统。该系统用于根据结核分枝杆菌生长过程中挥发性代谢产物NH3和CO2检测结核病。代谢产物从介质扩散到KOH吸收液中，导致MSPQC灵敏地检测到吸收液的电导变化。用自行开发的软件记录频移-时间响应曲线。以频移值为-100 Hz的频率检测时间(FDT)为参数，对结核分枝杆菌H37Ra菌株进行了定量检测。用该系统成功地检测了H37Ra和40株临床阳性标本。H37Ra在10(2)-10(7)个集落形成单位(CFU)ml(-1)范围内与其初始浓度的对数呈线性关系(R=-0.998)，检出限低至10CFU(-1)。用4%能杀灭污染微生物并能使结核分枝杆菌活着的氢氧化钠溶液作为前处理试剂，为该方法提供了选择性。还用BACTEC(TM)MGIT(TM)960和传统的洛温斯坦-詹森(L-J)斜板进行了对比试验。结果表明，该系统比BACTEC(TM)MGIT(TM)960更快，更便宜，将在世界范围内广泛应用于结核病检测。(C)2008爱思唯尔B.V.保留所有权利。
Many of the deaths caused by tuberculosis (TB) in the world are due to wrong or late diagnosis. The World Health Organization (WHO) calls for better and cheaper TB tests method for this reason. In this paper, a new multi-channel series piezoelectric quartz crystal (MSPQC) sensor system was developed for rapid growth and detection of Mycobacterium tuberculosis. It is an automatic continuous monitoring system. The system was used to detect TB based on the volatile metabolic products NH3 and CO2 during the growth of M. tuberculosis. The metabolic products, diffusing from the medium into the KOH absorbing solution, resulted in the conductance change of the absorbing solution detected by the MSPQC sensitively. The frequency shift versus time response curves were recorded by self-developed software. Frequency detection time (FDT) corresponding to -100 Hz in frequency shift value was used as a parameter to quantitatively determine M. tuberculosis H37Ra (an avirulent strain). H37Ra and 40 strains clinic positive samples were detected by the proposed system successfully. As for H37Ra, the FDT had a linear relationship with the logarithm of its initial concentration in the range of 10(2)-10(7) colony forming units (cfu) ml(-1) (R=-0.998) and the detection limit was low to 10 cfu ml(-1). 4% NaOH solution that can kill contaminating microorganisms and make M. tuberculosis alive was used as pretreatment reagent to provide selectivity to this method. Comparative tests were also carried out by using BACTEC (TM) MGIT (TM) 960 and conventional Lowenstein-Jensen (L-J) slants. The results showed that the proposed system was quicker than BACTEC (TM) MGIT (TM) 960 and it is also cheaper and will be widely used in TB tests in the world. (C) 2008 Elsevier B.V. All rights reserved.