The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato.
The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato.
复制标题
GAMYB 样基因 SlMYB33 介导番茄开花和花粉发育。
DOI：
10.1038/s41438-020-00366-1
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Liang Yan
中科院分区：
文献类型：
--
作者：
Zhang Yan;Zhang Bo;Yang Tongwen;Zhang Jie;Liu Bin;Zhan Xiangqiang;Liang Yan
gamyb是一种积极的遗传信号因子，在生殖发育，特别是花药和花粉发育中发挥重要作用。然而，没有直接证据表明GAMYB在番茄的这些生物过程中起调节作用。在这里，我们鉴定了一个类似于番茄gamyb的基因SlMYB33，并描述了它的具体作用。SlMYB33主要在雄蕊和雌蕊中表达。在花发育过程中，SlMYB33 mRNA在雄性和雌性器官，如小孢子母细胞、花药、花粉粒和胚珠中都检测到高丰度。SlMYB33基因的沉默导致番茄开花延迟、花粉活力异常和育性差。组织学分析表明，SlMYB33在花粉成熟阶段发挥其作用。进一步的转录组学分析表明，敲低SlMYB33显著抑制了茎尖开花相关基因的表达，并改变了花药中控制糖代谢的基因的转录。综上所述，我们的研究表明，SlMYB33可能通过调节分别负责开花和糖代谢的基因的表达来调节番茄的开花和花粉成熟度。
GAMYBs are positive GA signaling factors that exhibit essential functions in reproductive development, particularly in anther and pollen development. However, there is no direct evidence of the regulation of any GAMYB in these biological processes in tomato (Solanum lycopersicum). Here, we identified a tomato GAMYB-like gene, SlMYB33, and characterized its specific roles. SlMYB33 is predominately expressed in the stamens and pistils. During flower development, high mRNA abundance of SlMYB33 is detected in both male and female organs, such as microspore mother cells, anthers, pollen grains, and ovules. Silencing of SlMYB33 leads to delayed flowering, aberrant pollen viability, and poor fertility in tomato. Histological analyses indicate that SlMYB33 exerts its function in pollen development in the mature stage. Further transcriptomic analyses imply that the knockdown of SlMYB33 significantly inhibits the expression of genes related to flowering in shoot apices, and alters the transcription of genes controlling sugar metabolism in anthers. Taken together, our study suggests that SlMYB33 regulates tomato flowering and pollen maturity, probably by modulating the expression of genes responsible for flowering and sugar metabolism, respectively.