PCP and Wnt pathway components act in parallel during zebrafish mechanosensory hair cell orientation
PCP and Wnt pathway components act in parallel during zebrafish mechanosensory hair cell orientation
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DOI：
10.1038/s41467-019-12005-y
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发表时间：
2019-09-05
影响因子：
16.6
通讯作者：
Piotrowski, Tatjana
中科院分区：
文献类型：
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作者：
Acedo, Joaquin Navajas;Voas, Matthew G.;Piotrowski, Tatjana
平面细胞极性（PCP）在原肠胚形成、神经管闭合和听力等发育过程中起着至关重要的作用。由于其表型之间的相似性，Wnt 途径突变体通常被归类为 PCP 突变体。在这里，我们发现在斑马鱼侧线中，PCP 和 Wnt 通路的破坏对毛细胞方向有不同的影响。 PCP 基因 vangl2 和 scrib 的突变会导致毛细胞的随机方向，而 wnt11f1、gpc4 和 fzd7a/b 的突变则会诱导毛细胞采用同心模式。这种同心图案不是由 PCP 缺陷引起的，而是由于支持细胞未对准造成的。支持细胞缺陷的分子基础尚不清楚，但我们证明 PCP 和 Wnt 途径并行工作以建立正确的毛细胞方向。因此，毛细胞定向缺陷不仅仅可以用 PCP 信号传导缺陷来解释，而且一些毛细胞表型值得重新评估。
Planar cell polarity (PCP) plays crucial roles in developmental processes such as gastrulation, neural tube closure and hearing. Wnt pathway mutants are often classified as PCP mutants due to similarities between their phenotypes. Here, we show that in the zebrafish lateral line, disruptions of the PCP and Wnt pathways have differential effects on hair cell orientations. While mutations in the PCP genes vangl2 and scrib cause random orientations of hair cells, mutations in wnt11f1, gpc4 and fzd7a/b induce hair cells to adopt a concentric pattern. This concentric pattern is not caused by defects in PCP but is due to misaligned support cells. The molecular basis of the support cell defect is unknown but we demonstrate that the PCP and Wnt pathways work in parallel to establish proper hair cell orientation. Consequently, hair cell orientation defects are not solely explained by defects in PCP signaling, and some hair cell phenotypes warrant re-evaluation.