Molar concentration from sequential 2-D water-window X-ray ptychography and X-ray fluorescence in hydrated cells.
Molar concentration from sequential 2-D water-window X-ray ptychography and X-ray fluorescence in hydrated cells.
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DOI：
10.1038/srep24280
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发表时间：
2016-04-12
影响因子：
4.6
通讯作者：
van Riessen GA
中科院分区：
文献类型：
--
作者：
Jones MW;Elgass KD;Junker MD;de Jonge MD;van Riessen GA
生物X射线显微镜的最新发展，使结构信息和元素分布，同时获得结合X射线ptychography和X射线荧光显微镜。实验上，这些方法可以同时进行;然而，每个测量的最佳条件可能不兼容。在这里，我们结合了联合收割机两个不同的测量超微结构和元素分布，每一个测量优化条件下进行。通过结合优化的ptychography和荧光信息，我们能够从二维图像中确定摩尔浓度，从而可以调查成纤维细胞中的环境传感丝状伪足与细胞外钙之间的相互作用。此外，我们提出的生物ptychography结果说明了一个成熟点，该技术可以应用于解决结构生物学中的重大问题。
Recent developments in biological X-ray microscopy have allowed structural information and elemental distribution to be simultaneously obtained by combining X-ray ptychography and X-ray fluorescence microscopy. Experimentally, these methods can be performed simultaneously; however, the optimal conditions for each measurement may not be compatible. Here, we combine two distinct measurements of ultrastructure and elemental distribution, with each measurement performed under optimised conditions. By combining optimised ptychography and fluorescence information we are able to determine molar concentrations from two-dimensional images, allowing an investigation into the interactions between the environment sensing filopodia in fibroblasts and extracellular calcium. Furthermore, the biological ptychography results we present illustrate a point of maturity where the technique can be applied to solve significant problems in structural biology.