Use of both direct and indirect 13C tags for probing nitrogen interactions in hairpin ribozyme models by 15N NMR.
Use of both direct and indirect 13C tags for probing nitrogen interactions in hairpin ribozyme models by 15N NMR.
复制标题
使用直接和间接 13C 标签通过 15N NMR 探测发夹核酶模型中的氮相互作用。
DOI：
10.1081/ncn-120027834
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Jones,RogerA
中科院分区：
文献类型：
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作者：
Shallop,AnthonyJ;Gaffney,BarbaraL;Jones,RogerA
We have used the synthesis and15N NMR study of separate loop A and loop B domains of the hairpin ribozyme to demonstrate that multiple15N atoms can be incorporated into an RNA strand and be unambiguously distinguished through a combination of direct and indirect tagging by13C atoms. Absence of15N chemical shift changes shows that the G8N1 in loop A does not become deprotonated up to pH 8, and that the G21N7 of loop B does not bind to Mg2 +.
