Syntheses and properties of tertiary peptide bond-containing polypeptides.
Syntheses and properties of tertiary peptide bond-containing polypeptides.
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含三级肽键的多肽的合成和性质。
DOI：
10.1111/j.1399-3011.1984.tb02725.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
H. Nakano
中科院分区：
文献类型：
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作者：
M. Narita;Tsutomu Fukunaga;Atsushi Wakabayashi;K. Ishikawa;H. Nakano
采用逐步延伸法和片段缩合法制备了含有Pro或（Dmob）Leu残基的寡聚（Leu）。制备的肽如下：博克-莱昂-奥布兹尔（n = 3-6和9）、Boc-Pro-Leu3-OBzl、Boc-Leun-Pro-OBzl（n = 3-5）、Boc-Leun-Pro-Leu3-OBzl（n = 3和4），Boc-（Leu4-Pro）2-OBzl、Boc-Leu3-Pro-Leun-Pro-Leu3-OBzl（n = 6和7）、Boc-Leu3-Gly-（Dmob）Leu-OMe、Boc-[Leu3-Gly-（Dmob）Leu-Leu3]n-OBzl（n = 1和2）。将Pro和Gly-（Dmob）Leu残基插入到肽链中引起“肽段分离”，并实现了N-和C-保护的寡（Leu）衍生物在各种有机溶剂中的溶解度的显著改善。还使用i.r.研究了典型肽在固态和DMSO中的构象。谱在β-折叠结构的中心位置引入三级肽键诱导固态中无序结构的开始。在DMSO中，肽有效地经受溶剂化以具有无序结构。
Oligo(Leu)s containing Pro or (Dmob) Leu residues were prepared by the stepwise elongation and fragment condensation methods. The peptides prepared were the following: Boc-Leun-OBzl (n = 3–6 and 9), Boc-Pro-Leu3-OBzl, Boc-Leun-Pro-OBzl (n = 3–5), Boc-Leun-Pro-Leu3-OBzl (n = 3 and 4), Boc-(Leu4-Pro)2-OBzl, Boc-Leu3-Pro-Leun-Pro-Leu3-OBzl (n = 6 and 7), Boc-Leu3-Gly-(Dmob) Leu-OMe, Boc-[Leu3-Gly-(Dmob) Leu-Leu3]n-OBzl (n = 1 and 2). The insertion of the Pro and Gly-(Dmob) Leu residues into a peptide chain caused “the peptide segment separation” and achieved remarkable solubility improvement of N- and C-protected oligo(Leu) derivatives in a variety of organic solvents. The conformations of typical peptides in the solid state and in DMSO were also investigated using i.r. spectroscopy. The incorporation of tertiary peptide bonds in a central position of the β-sheet structure induces the onset of an unordered structure in the solid state. In DMSO, peptides are efficiently subjected to solvation to have the unordered structure.