Zebrafish fin regeneration after cryoinjury-induced tissue damage.
Zebrafish fin regeneration after cryoinjury-induced tissue damage.
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DOI：
10.1242/bio.016865
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发表时间：
2016-06-15
期刊：
影响因子：
2.4
通讯作者：
Jaźwińska A
中科院分区：
文献类型：
--
作者：
Chassot B;Pury D;Jaźwińska A
尽管截肢手术后的鱼鳍再生已经有了很好的描述，但对斑马鱼附属物中长时间组织损伤对再生程序执行的影响知之甚少。为了诱导尾鳍的组织溶解过程，我们建立了一种新的冷冻损伤模型，该模型结合了冷冻/解冻和缺血的有害影响。与普通横断模型相比，冻伤后鳍的受损部分在2天内自发脱落。剩下的残肢包含一个扭曲的边缘，其中混合了死亡物质和健康细胞，同时分别诱导了组织碎片降解和细胞增殖两个相反的过程。在冷冻损伤后2 - 7天，这个修复/增殖阶段的形态学特征是骨折碎片移位。在残端边缘下方完整的间质中诱导一个胚基标记物msxB。上皮细胞和成骨细胞转基因报告系的实时成像显示，组织特异性再生程序是在清除受损物质后启动的。与单纯截肢模型相比，尽管在冷冻损伤后的第一周有组织溶解性扰动，但鳍再生恢复并完成，没有进一步的改变。该模型揭示了斑马鱼在残肢长时间组织分解后恢复原始附属物结构的强大能力。总结：鱼鳍冻裂导致组织溶解和延迟性组织丢失。尽管长时间破坏残肢结构，但鳍再生恢复并正常完成，显示再生能力的稳健性。
Although fin regeneration following an amputation procedure has been well characterized, little is known about the impact of prolonged tissue damage on the execution of the regenerative programme in the zebrafish appendages. To induce histolytic processes in the caudal fin, we developed a new cryolesion model that combines the detrimental effects of freezing/thawing and ischemia. In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury. The remaining stump contained a distorted margin with a mixture of dead material and healthy cells that concomitantly induced two opposing processes of tissue debris degradation and cellular proliferation, respectively. Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones. A blastemal marker msxB was induced in the intact mesenchyme below the damaged stump margin. Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material. Despite histolytic perturbation during the first week after cryoinjury, the fin regeneration resumed and was completed without further alteration in comparison to the simple amputation model. This model reveals the powerful ability of the zebrafish to restore the original appendage architecture after the extended histolysis of the stump. Summary: Fin cryolesion resulted in histolysis and a delayed tissue loss. Despite prolonged destruction of the stump architecture, fin regeneration resumed and was normally completed, revealing robustness of the regenerative capacity.