Biodegradable zinc oxide composite scaffolds promote osteochondral differentiation of mesenchymal stem cells
Biodegradable zinc oxide composite scaffolds promote osteochondral differentiation of mesenchymal stem cells
复制标题
DOI：
10.1002/bit.27173
复制
发表时间：
2020-01-01
影响因子：
3.8
通讯作者：
Arinzeh, Treena Livingston
中科院分区：
文献类型：
--
作者：
Khader, Ateka;Arinzeh, Treena Livingston
骨关节炎（OA）是一种累及关节软骨和软骨下骨的退行性病变。关节软骨修复和再生的能力是有限的。制备了一种可生物降解的含氧化锌（ZnO）的纤维支架，并对其在骨软骨组织工程中的应用进行了评价。ZnO在多种生物医学应用中显示出了前景，但在组织工程中的应用有限。复合支架由嵌入缓慢降解的聚己内酯中的ZnO纳米颗粒组成，以允许锌离子随时间溶解。锌具有众所周知的胰岛素模拟特性，并且可以有益于软骨和骨再生。纤维状ZnO复合支架，具有1-10重量%的不同浓度氧化锌，使用静电纺丝技术制备和评估人骨髓间充质干细胞（MSC）分化沿着软骨细胞和成骨细胞谱系。观察到所有ZnO复合支架的锌的缓慢释放。MSC成软骨分化促进低百分比ZnO复合支架上的最高II型胶原蛋白的生产和软骨特异性基因的表达，而成骨分化促进高百分比ZnO复合支架上的最高碱性磷酸酶活性，胶原蛋白的生产，和骨特异性基因的表达。本研究表明，含氧化锌的复合材料作为骨软骨组织工程的潜在支架的可行性。
Osteoarthritis (OA) involves the degeneration of articular cartilage and subchondral bone. The capacity of articular cartilage to repair and regenerate is limited. A biodegradable, fibrous scaffold containing zinc oxide (ZnO) was fabricated and evaluated for osteochondral tissue engineering applications. ZnO has shown promise for a variety of biomedical applications but has had limited use in tissue engineering. Composite scaffolds consisted of ZnO nanoparticles embedded in slow degrading, polycaprolactone to allow for dissolution of zinc ions over time. Zinc has well-known insulin-mimetic properties and can be beneficial for cartilage and bone regeneration. Fibrous ZnO composite scaffolds, having varying concentrations of 1-10 wt.% ZnO, were fabricated using the electrospinning technique and evaluated for human mesenchymal stem cell (MSC) differentiation along chondrocyte and osteoblast lineages. Slow release of the zinc was observed for all ZnO composite scaffolds. MSC chondrogenic differentiation was promoted on low percentage ZnO composite scaffolds as indicated by the highest collagen type II production and expression of cartilage-specific genes, while osteogenic differentiation was promoted on high percentage ZnO composite scaffolds as indicated by the highest alkaline phosphatase activity, collagen production, and expression of bone-specific genes. This study demonstrates the feasibility of ZnO-containing composites as a potential scaffold for osteochondral tissue engineering.