ANALYSIS OF KINETIC DATA FOR IRREVERSIBLE ENZYME-INHIBITION
ANALYSIS OF KINETIC DATA FOR IRREVERSIBLE ENZYME-INHIBITION
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DOI：
10.1042/bj2570419
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发表时间：
1989-01-15
影响因子：
4.1
通讯作者：
DUGGLEBY, RG
中科院分区：
文献类型：
--
作者：
GRAY, PJ;DUGGLEBY, RG
许多有机磷化合物是乙酰胆碱酯酶的不可逆抑制剂。文献中用于确定缓蚀动力学常数的方法通常包括手动确定缓蚀过程曲线上各点的斜率或对曲线拟合多项式。本研究探讨了使用非线性回归分析来确定各种参数。提出了一种方法，可以在一定范围的情况下得到精确的抑制常数值。
Many organophosphorus compounds are irreversible inhibitors of acetylcholinesterase. The methods used in the literature to determine the inhibition kinetic constants usually involve either manual determination of the slope at various points along the inhibition progress curve or fitting polynomials to the curve. The present study investigates the use of non-linear-regression analysis to determine the various parameters. A method is suggested that yields accurate values for the inhibition constants under a range of circumstances.